JoVE Logo

Sign In

Seoul St. Mary's Hospital, Institute of Medical Science, Republic of Korea

5 ARTICLES PUBLISHED IN JoVE

image

Developmental Biology

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
Yeri Alice Rim 1,3, Narae Park 1, Yoojun Nam 1, Ji Hyeon Ju 2,3
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, Republic of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, Republic of Korea, 3College of Medicine, The Catholic University of Korea, Republic of Korea

Here we describe a protocol for generating human induced-pluripotent stem cells from patient-derived fibroblast-like synoviocytes, using a lentiviral system without feeder cells.

image

Developmental Biology

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
Yeri Alice Rim 1, Yoojun Nam 1, Ji Hyeon Ju 2
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.

image

Developmental Biology

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
Yeonsue Jang 1, Hyerin Jung 1, Ji Hyeon Ju 1
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea

Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

image

Developmental Biology

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
Yoojun Nam 1, Yeri Alice Rim 1, Ji Hyeon Ju 2
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

Here, we propose a protocol for chondrogenic differentiation from cord blood mononuclear cell-derived human induced pluripotent stem cells.

image

Developmental Biology

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Yena Kim 1,2, Ji Hyeon Ju 1,2,3
1CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine, The Catholic University of Korea, 2Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 3Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved