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The Chinese University of Hong Kong, Hong Kong Special Administrative Region

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
Wai Wa Ray Chan *1, Wen Li *1,2, Dik Long Dennis Chau *1, Kwok-Fai Lau 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen

In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

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Biochemistry

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
Wai Wa Ray Chan *1, Yu Qi Zhai *1, Kwok-Fai Lau 1
1School of Life Sciences, The Chinese University of Hong Kong

This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.

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