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3 ARTICLES PUBLISHED IN JoVE

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Biology

Immunoblot Analysis
Sean Gallagher 1, Deb Chakavarti 2
1UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.

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Biology

Staining Proteins in Gels
Sean Gallagher 1, Deb Chakavarti 2
1Keck Graduate Institute of Applied Life Sciences, UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences

Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.

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Cancer Research

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
Jonathan A. Moreno 1, Antonio Sanchez 2, Robert M. Hoffman 3,4, Saima Nur 1, Maria P. Lambros 1
1College of Pharmacy, Western University of Health Sciences, 2UVP, LLC, 3Department of Surgery, School of Medicine, University of California San Diego, 4Anticancer Inc.

A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.

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