Immunoblot Analysis

68.7K Views

16:01 min

June 20th, 2008

DOI :

10.3791/759-v

June 20th, 2008


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Immunoblot Analysis

Chapters in this video

0:00

Title/ Prologue

0:56

Introduction

1:22

Separating Protein Samples by Gel Electrophoresis

2:06

Assembling the Immunoblot Sandwich

5:43

Transferring Proteins from Gel to Membrane

7:52

Immunoprobing Membrane with Directly Conjugated Secondary Antibody

10:45

Immunoprobing with Avidin-Biotin Coupling to Secondary Antibody

14:26

Visualization of Immunoblot with Chromogenic Substrates

15:32

Conclusion

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