JoVE Logo

Sign In

University of Oklahoma

6 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Electrophysiology of Scorpion Peg Sensilla
Elizabeth D. Knowlton 1, Douglas D. Gaffin 1
1Department of Zoology, University of Oklahoma

This article describes an electrophysiological method for isolating chemical stimulation to individual sensilla via extracellular, tip-recordings under mineral oil.

image

Biology

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
Wei Rao 1, Ning Pan 1, Zhibo Yang 1
1Department of Chemistry and Biochemistry, University of Oklahoma

Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.

image

Bioengineering

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
Christopher A. Neal 1, Jennifer G. Nelson-Brantley 1, Michael S. Detamore 2, Hinrich Staecker 1, Adam J. Mellott 3
1Department of Otolaryngology, University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering, University of Oklahoma, 3Department of Plastic Surgery, University of Kansas Medical Center

The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications.

image

Engineering

Magnet Assisted Composite Manufacturing: A Flexible New Technique for Achieving High Consolidation Pressure in Vacuum Bag/Lay-Up Processes
Maya Pishvar *1, Mehrad Amirkhosravi *1, M. Cengiz Altan 1
1School of Aerospace and Mechanical Engineering, University of Oklahoma

A new technique for applying consolidation pressure on the vacuum bag lay-up to fabricate composite laminates is described. The goal of this protocol is to develop a simple, cost-effective technique to improve the quality of laminates fabricated by the wet lay-up vacuum bag method.

image

Chemistry

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Shawna J. Standke 1, Devon H. Colby 1, Ryan C. Bensen 1, Anthony W. G. Burgett 1, Zhibo Yang 1
1Department of Chemistry and Biochemistry, University of Oklahoma

An integrated cell manipulation platform is developed for use in conjunction with a single-probe mass spectrometry setup for the on-line analysis of individual suspension cells under ambient conditions.

image

Environment

In Situ Soil Moisture Sensors in Undisturbed Soils
Todd G. Caldwell 1, Michael H. Cosh 2, Steven R. Evett 3, Nathan Edwards 4, Heather Hofman 5, Bradley G. Illston 6, Tilden Meyers 7, Marina Skumanich 8, Kent Sutcliffe 9
1Nevada Water Science Center, U.S. Geological Survey, 2Agricultural Research Service, Hydrology and Remote Sensing Lab., U.S. Department of Agriculture, 3Agricultural Research Service, Conservation and Production Research Lab., U.S. Department of Agriculture, 4South Dakota State University, 5National Water and Climate Center, U.S. Department of Agriculture, 6University of Oklahoma, 7Air Resources Lab, National Oceanic and Atmospheric Administration, 8National Integrated Drought Information System, National Oceanic and Atmospheric Administration, 9Snow Survey Staff, U.S. Department of Agriculture

The determination of soil water content is a critical mission requirement for many state and federal agencies. This protocol synthesizes multi-agency efforts to measure soil water content using buried in situ sensors.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved