University of Shizuoka

1 ARTICLES PUBLISHED IN JoVE

Biology

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

Wendy Hempstock¹, Noriko Ishizuka¹, Hisaysohi Hayashi¹

¹Laboratory of Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka

Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.