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Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique

4.9K Views

06:43 min

May 26th, 2021

May 26th, 2021

4,912 Views

0:04

Introduction

0:55

Stripping the Muscle Layer and Preparing the Intestinal Sheet

2:12

Mounting Intestinal Preparations in Ussing Chambers

3:05

Measurement of Dilution Potential (Open Circuit Conditions)

4:07

Transepithelial Electrical Conductance and Baseline Isc (Short-Circuit Conditions)

4:55

Results: Transepithelial Electrical Conductance and Dilution Potential in Cldn15-/- Mice

6:12

Conclusion

Transcript

This protocol is used to study the transport qualities of tight junctions. Using dilution potentials, you can measure the permselectivity and get an understanding of tight junctions in a tissue. This technique is specific and uses native tissues f

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Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.

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