JoVE Logo

Sign In

University of Venda

1 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
Hloniphani Rachel Ncube 1, Unarine Dali 1, Tendamudzimu Harmfree Dongola 1, Addmore Shonhai 1
1Department of Biochemistry & Microbiology, University of Venda

This protocol demonstrates the chaperone activity of heat shock protein 70 (Hsp70). E. coli dnaK756 cells serve as a model for the assay as they harbor a native, functionally impaired Hsp70, making them susceptible to heat stress. The heterologous introduction of functional Hsp70 rescues the growth deficiency of the cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved