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University of Verona, Verona, Italy

3 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
Elisa Gecchele *1, Matilde Merlin *1, Annalisa Brozzetti 2, Alberto Falorni 2, Mario Pezzotti 1, Linda Avesani 1
1Department of Biotechnology, University of Verona, Verona, Italy, 2Department of Internal Medicine, University of Perugia, Perugia, Italy

In this study the expression of a target human recombinant protein in different production platforms was compared. We focused on traditional fermenter-based cultures and on plants, describing the set-up of each system and highlighting, on the basis of the reported results, the inherent limits and advantages for each platform.

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Genetics

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Silvia Dal Santo *1, Mauro Commisso *1, Erica D'Incà 1, Andrea Anesi 1,2, Matteo Stocchero 3, Sara Zenoni 1, Stefania Ceoldo 1, Giovanni B. Tornielli 1, Mario Pezzotti 1, Flavia Guzzo 1
1Biotechnology Department, University of Verona, 2Lab. of Bioorganic Chemistry, Physics Department, University of Trento, 3S-IN Soluzioni Informatiche

This article describes the application of untargeted metabolomics, transcriptomics and multivariate statistical analysis to grape berry transcripts and metabolites in order to gain insight into the terroir concept, i.e., the impact of the environment on berry quality traits.

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Bioengineering

Transient Expression in Red Beet of a Biopharmaceutical Candidate Vaccine for Type-1 Diabetes
Mattia Santoni *1, Edoardo Bertini *1, Roberta Zampieri 1, Anna Cuccurullo 1, Mauro Commisso 1, Elisa Gecchele 1, Linda Avesani 1
1Department of Biotechnology, University of Verona

Here, we present a protocol to produce an oral vaccine candidate against Type 1 diabetes in an edible plant.

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