Weill Cornell Medicine

5 ARTICLES PUBLISHED IN JoVE

Cancer Research

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

George Zhang¹, Yudan Chi¹, Yi-Chieh Nancy Du¹

¹Department of Pathology and Laboratory Medicine, Weill Cornell Medicine

We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.

Engineering

Rapid Manufacturing of Thin Soft Pneumatic Actuators and Robots

Amir Ali Amiri Moghadam^1,2,3, Alexandre Caprio^1,2, Seyedhamidreza Alaie^1,2, James K. Min^1,2, Simon Dunham^1,2, Bobak Mosadegh^1,2

¹Dalio Institute of Cardiovascular Imaging, New York-Presbyterian Hospital, Weill Cornell Medicine, ²Department of Radiology, Weill Cornell Medicine, ³Mechatronics Engineering Department, Kennesaw State University

This protocol describes a method for rapid manufacturing of soft pneumatic actuators and robots with a thin form factor. The fabrication method starts with lamination of thermoplastic polyurethane (TPU) sheets followed by laser cutting/welding of a two-dimensional pattern to form actuators and robots.

Developmental Biology

Intramyocardial Injection for the Study of Cardiac Lymphatic Function in Zebrafish

Laila Abd Elmagid^*1,2, Nishant Mittal^*1,2, Isaac Bakis^1,2, Ching-Ling Lien³, Michael R. Harrison^1,2

¹Cardiovascular Research Institute, Weill Cornell Medicine, ²Department of Cell and Developmental Biology, Weill Cornell Medicine, ³The Saban Research Institute and Heart Institute, Children's Hospital Los Angeles

The present protocol allows efficient and stable delivery of fluorescent microspheres (MS) and quantum dots (QD) into the myocardium of live fish that can be tracked (traced) over time.

Cellular And Small Animal Models Of Neurodegenerative Diseases

Jacqueline Burré¹, Manu Sharma¹

¹Appel Alzheimer’s Disease Research Institute, Brain and Mind Research Institute, Weill Cornell Medicine

Cellular And Small Animal Models Of Neurodegenerative Diseases

Biology

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Pania E. Bridge-Comer¹, Shannon M. Reilly¹

¹Weill Center for Metabolic Health, Department of Medicine, Weill Cornell Medicine

Triglyceride lipolysis in adipocytes is an important metabolic process resulting in the liberation of free fatty acids and glycerol. Here, we provide a detailed protocol to measure basal and stimulated lipolysis in adipocytes and ex vivo adipose tissue from mice.