Heinrich-Heine-University Dusseldorf
2 ARTICLES PUBLISHED IN JoVE
Biology
An Innovative Method for Exosome Quantification and Size Measurement
Arash Mehdiani 1, Anatol Maier 1, Antonio Pinto 1, Mareike Barth 1, Payam Akhyari 1, Artur Lichtenberg 1
1Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf
A method for isolation of exosomes from whole blood and further analysis by nanoparticle tracking using a semi-automatic instrument is presented in this article. The presented technology provides an extremely sensitive method for visualizing and analyzing particles in liquid suspension.
Biochemistry
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
Andreas Weber 1, Julia Christin Wehmeyer 1, Vera Schmidt 1, Artur Lichtenberg 1, Payam Akhyari 1
1Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University
In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.
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