Accedi

University of Chinese Academy of Science

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Manju Sharma¹, Xiang Zhang¹, Shouxiong Huang¹

¹Department of Environmental Health, University of Cincinnati College of Medicine

Imaging flow cytometry provides an ideal approach to detect the morphological and functional alteration of cells at individual and populational levels. Disrupted endocytic function for lipid antigen presentation in pollutant-exposed human dendritic cells was demonstrated with a combined transcriptomic profiling of gene expression and morphological demonstration of protein trafficking.

Engineering

Graphene-Assisted Quasi-van der Waals Epitaxy of AlN Film on Nano-Patterned Sapphire Substrate for Ultraviolet Light Emitting Diodes

Xiang Zhang^*1,2, Zhaolong Chen^*3, Hongliang Chang^1,2, Jianchang Yan^1,2, Shenyuan Yang^2,4, Junxi Wang^1,2, Peng Gao⁵, Tongbo Wei^1,2

¹State Key Laboratory of Solid-State Lighting, Institute of Semiconductors, Chinese Academy of Sciences, ²Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, ³Center for Nanochemistry (CNC), Beijing National Laboratory for Molecular Science, College of Chemistry and Molecular Engineering, Peking University, ⁴State Key Laboratory of Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences, ⁵Electron Microscopy Laboratory, School of Physics, Peking University

A protocol for graphene-assisted growth of high-quality AlN films on nano-patterned sapphire substrate is presented.