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Medical University of Graz, Austria

3 ARTICLES PUBLISHED IN JoVE

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Biology

Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Katharina Schallmoser 1, Dirk Strunk 1
1Stem Cell Research Unit, Medical University of Graz, Austria

Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.

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Biology

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
Nicole A. Hofmann 1, Andreas Reinisch 1, Dirk Strunk 1
1Stem Cell Research Unit, Medical University of Graz, Austria

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

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Biology

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
Andreas Reinisch 1, Dirk Strunk 1
1Stem Cell Research Unit, Medical University of Graz, Austria

This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.

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