Sign In

A subscription to JoVE is required to view this content.

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

10.5K Views

12:17 min

October 28th, 2009

October 28th, 2009

10,514 Views

0:00

Title

0:41

Introduction

1:18

Isolation of Endothelial Colony-Forming Progenitor Cells (ECFCs)

5:46

Harvesting the ECFCs

8:19

ECFC Expansion

10:57

Representative ECFC Preparations

11:45

Conclusion

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More