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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

DOI :

10.3791/1524-v

October 28th, 2009

October 28th, 2009

10,429 Views

1Stem Cell Research Unit, Medical University of Graz, Austria

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

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Isolation

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