Preparing the CaspaseTracker Flies and Dissected Tissues
4:30
Tissue Staining and Mounting
8:11
Confocal Microscopy
9:37
Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
12:10
Conclusion
Trascrizione
The overall goal of this experiment is to detect and track non-apoptotic caspase activity in live animals using Drosophila Melanogaster as a model. Caspases are proteases that cleave many cellular components to cause apoptotic cell death. Therefor
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To detect healthy cells in whole animals that contain low levels of caspase activity, the highly sensitive biosensor designated CaspaseTracker was generated for Drosophila. Caspase-dependent biosensor activity is detected in long-lived healthy cells throughout the internal organs of adult animals reared under optimized conditions in the absence of death stimuli.