Induction and Establishment of Tetraploid Cells from Diploid Fibroblasts without the Shake-off Method
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Induction and Establishment of Tetraploid Cells from Diploid Fibroblasts with the Shake-off Method
4:01
Examination of DNA Ploidy
6:30
Preparation for Chromosome Counts and Karyotype Analysis
8:04
Results: Establishment of Proliferative Tetraploid Cells
9:28
Conclusion
Trascrizione
The overall goal of this procedure is to establish proliferative tetraploid cells, free of a diploid population, from normal human fibroblasts. This method can help answer key questions in the field of carcinogenesis research, such as how aneuploi
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Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.