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Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Journal (Cancer Research)

Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells

Carli Stewart¹^,²^,³, Claudia Manriquez Roman¹^,⁴, Saad S. Kenderian¹^,⁵^,⁶

¹T Cell Engineering, Mayo Clinic, ²Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, ³Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, ⁴Center for Regenerative Biotherapeutics, Mayo Clinic, ⁵Division of Hematology, Mayo Clinic, ⁶Department of Immunology, Mayo Clinic

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Journal (Cancer Research) | Methods Collections

A Nonviral Approach to Generate Transient Chimeric Antigen Receptor T Cells Using mRNA for Cancer Immunotherapy

Liang Hu¹, Robert Berahovich¹, Yanwei Huang¹, Shiming Zhang¹, Jinying Sun¹, Xianghong Liu¹, Hua Zhou¹, Shirley Xu¹, Haoqi Li¹, Vita Golubovskaya¹, Lijun Wu¹

¹ProMab Biotechnologies

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Journal (Cancer Research) | Methods Collections

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Maryna Somova¹, Marlene Grosse¹, Frank Schulze², Martin Burchardt¹, Pedro Caetano Pinto¹

¹Department of Urology, University Medicine Greifswald, ²Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Journal (Cancer Research) | Methods Collections

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

The Huong Chau¹, Naaz Bansal¹, Anastasia Chernykh¹, Rebeca Kawahara¹^,², Morten Thaysen-Andersen¹^,²

¹School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, ²Institute for Glyco-core Research (iGCORE), Nagoya University

Preclinical Positron Emission Tomography with Body Conforming Animal Molds for Cloud-Based Automated Image Analysis in Mice

Journal (Cancer Research)

Preclinical Positron Emission Tomography with Body Conforming Animal Molds for Cloud-Based Automated Image Analysis in Mice

Edward Cabral¹, Mary Katherine Montgomery¹, Meaghan Berg², Lisa Kathryn Manzuk², Anand Giddabasappa¹, Ziyue Karen Jiang¹

¹Drug Safety R&D, Discovery & Early Development, Pfizer, Inc., ²Comparative Medicine, Discovery & Early Development, Pfizer, Inc.

Flow Cytometry of Hoechst-Stained Mouse Bone Marrow Cells for Effective Detection of Side Population Using High-Power 375 nm and 405 nm Lasers

Journal (Cancer Research)

Flow Cytometry of Hoechst-Stained Mouse Bone Marrow Cells for Effective Detection of Side Population Using High-Power 375 nm and 405 nm Lasers

Fangfang Wang¹^,², Xinyu Zhai¹, Tingting Guo³, Hengyi Xiao², Jingcao Huang¹

¹Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ²The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, ³Precision Medicine Research Laboratory, West China Hospital, Sichuan University

Staining Mouse Bone Marrow Cells with Hoechst to Detect Side Population Cells by Flow Cytometry

Journal (Cancer Research)

Staining Mouse Bone Marrow Cells with Hoechst to Detect Side Population Cells by Flow Cytometry

Fangfang Wang¹^,², Xinyu Zhai¹, Tingting Guo³, Hengyi Xiao², Jingcao Huang¹

¹Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ²The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, ³Precision Medicine Research Laboratory, West China Hospital, Sichuan University

Ex Vivo Fluorescence Microscopy of Murine Brain Tissue Sections

Journal (Cancer Research)

Ex Vivo Fluorescence Microscopy of Murine Brain Tissue Sections

Bryan D. Kim¹^,², Sujan K. Mondal¹^,³, Elizabeth Kenyon¹^,³, Ming Chen¹^,³, Christiane L. Mallett³^,⁴, Ana C. deCarvalho⁵, Zdravka Medarova⁶, Anna Moore¹^,³

¹Precision Health Program, Michigan State University, ²Department of Biomedical Engineering, College of Engineering, Michigan State University, ³Department of Radiology, College of Human Medicine, Michigan State University, ⁴Institute for Quantitative Health Science and Engineering, Michigan State University, ⁵Henry Ford Health, ⁶Transcode Therapeutics Inc.

MN-anti-miR10b Administration in Murine Glioblastoma Models for Bioluminescence and Fluorescence Imaging

Journal (Cancer Research)

MN-anti-miR10b Administration in Murine Glioblastoma Models for Bioluminescence and Fluorescence Imaging

Bryan D. Kim¹^,², Sujan K. Mondal¹^,³, Elizabeth Kenyon¹^,³, Ming Chen¹^,³, Christiane L. Mallett³^,⁴, Ana C. deCarvalho⁵, Zdravka Medarova⁶, Anna Moore¹^,³

¹Precision Health Program, Michigan State University, ²Department of Biomedical Engineering, College of Engineering, Michigan State University, ³Department of Radiology, College of Human Medicine, Michigan State University, ⁴Institute for Quantitative Health Science and Engineering, Michigan State University, ⁵Henry Ford Health, ⁶Transcode Therapeutics Inc.

In Vivo and Ex Vivo Imaging for Nanoparticle Tracking in Murine Glioblastoma Models

Journal (Cancer Research)

In Vivo and Ex Vivo Imaging for Nanoparticle Tracking in Murine Glioblastoma Models

Bryan D. Kim¹^,², Sujan K. Mondal¹^,³, Elizabeth Kenyon¹^,³, Ming Chen¹^,³, Christiane L. Mallett³^,⁴, Ana C. deCarvalho⁵, Zdravka Medarova⁶, Anna Moore¹^,³

¹Precision Health Program, Michigan State University, ²Department of Biomedical Engineering, College of Engineering, Michigan State University, ³Department of Radiology, College of Human Medicine, Michigan State University, ⁴Institute for Quantitative Health Science and Engineering, Michigan State University, ⁵Henry Ford Health, ⁶Transcode Therapeutics Inc.

Rapid Dissociation of Tumor Tissue to Gain Enhanced Single-Cell Tumor Insights

Journal (Cancer Research)

Rapid Dissociation of Tumor Tissue to Gain Enhanced Single-Cell Tumor Insights

Jacy Fang*¹^,², Isabella Salinas*¹^,², Sarah San Vicente*¹^,², Caroline Zielinski*¹^,², Moshe Sade-Feldman¹^,²^,³

¹Krantz Family Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³Department of Medicine, Harvard Medical School

Red Blood Cell Lysis in Digested Tumor Samples for Single-Cell RNA Sequencing

Journal (Cancer Research)

Red Blood Cell Lysis in Digested Tumor Samples for Single-Cell RNA Sequencing

Jacy Fang*¹^,², Isabella Salinas*¹^,², Sarah San Vicente*¹^,², Caroline Zielinski*¹^,², Moshe Sade-Feldman¹^,²^,³

¹Krantz Family Center for Cancer Research, Department of Medicine, Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³Department of Medicine, Harvard Medical School

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