3' Barcoded Adapter Ligations with 18 Individual RNA Samples
2:00
Ligated Small RNA Purification
5:26
5' Ligated and 3' Barcoded Purified Small RNAs Reverse Transcription
8:12
Pilot PCR and Large-scale PCR Amplification
10:46
Results: Protocol Robust Whether Fresh, Frozen, or Aged
12:01
Conclusion
Trascrizione
The overall goal of this CDNA Library preparation is to enable successful sequencing of small RNAs that have been stored in formal and fixed paraffin-embedded tissues. This method is very useful for analyzing formal and fixed paraffin-embedded spe
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Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.