Accedi

Hackensack University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Genetics

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Olivier Loudig^1,2,3, Christina Liu^1,2, Thomas Rohan³, Iddo Z. Ben-Dov⁴

¹Department of Research, Hackensack University Medical Center, ²Department of Medical Sciences, Seton Hall University, ³Department of Epidemiology and Population Health, Albert Einstein College of Medicine, ⁴Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.

Immunology and Infection

A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice

Michael T. McGuire^*1, Andrea Z. Tuckett^*2, Faith Myint², Johannes L. Zakrzewski^2,3,4

¹Department of Radiology, Hackensack University Medical Center, ²Center for Discovery and Innovation, Hackensack Meridian Health, ³Department of Pediatrics, Hackensack University Medical Center, ⁴Department of Oncology, Georgetown University

The present protocol describes an interventional radiology procedure established for intrathymic injection in mice to avoid the risk of open surgery and improve the accuracy of blind percutaneous injections.