Set-up of the CZE-MS/MS System and Analysis of the Samples
6:45
Database Search of the Collected Raw Files with the TopPIC Software
8:05
Results: Identification and Characterization of Proteoforms
9:14
Conclusion
Trascrizione
This method can help improve large scale and high resolution characterization of protein isoforms and protein post-translational modifications in cells. The main advantage of this technique is that capillary zone electrophoresis mass spectrometry
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A detailed protocol is described for the separation, identification, and characterization of proteoforms in protein samples using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS). The protocol can be used for the high-resolution characterization of proteoforms in simple protein samples and the large-scale identification of proteoforms in complex proteome samples.