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Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

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12:19 min

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April 22nd, 2022

DOI :

10.3791/63089-v

April 22nd, 2022

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3,629 Views

Hui-Lan Chang^1*, Kang-Yi Su^1,2*, Steven D. Goodman³, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Sui-Yuan Chang^1,2, Woei-horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University

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Keywords Uracil DNA Glycosylase

MALDI TOF Mass Spectrometry

Enzyme Activity Assay

Guanine uracil Base

Reaction Buffer

Substrate Preparation

Reaction Termination

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