This protocol allows resolving the membrane periodic skeleton in the axon initial segment of cultured neurons. By examining the localization of proteins insight can be gained into the function in the membrane periodic skeleton. The main advantage
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The present protocol describes a method to visualize and measure actin rings and other components of the membrane periodic skeleton of the axon initial segment using cultured rat hippocampal neurons and 3D-structured illumination microscopy (3D-SIM).