Paul W. Wiseman

Image correlation spectroscopy: mapping correlations in space, time, and reciprocal space.

Isolation of bright aggregate fluctuations in a multipopulation image correlation spectroscopy system using intensity subtraction.

Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microscopy.

Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells.

Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Measuring fast dynamics in solutions and cells with a laser scanning microscope.

Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy.

Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy.

Two-photon fluorescent microlithography for live-cell imaging.

Membrane lateral diffusion and capture of CFTR within transient confinement zones.

k-Space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore photophysics.

Measurement of monomer-oligomer distributions via fluorescence moment image analysis.

A guide to accurate fluorescence microscopy colocalization measurements.

Probing the integrin-actin linkage using high-resolution protein velocity mapping.

Investigating membrane protein dynamics in living cells.

Src-dependent phosphorylation of beta2-adaptin dissociates the beta-arrestin-AP-2 complex.

Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules.

Fabrication of protein gradients for cell culture using a miniature squeegee.

Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility.

Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells.

Sensitive detection of malaria infection by third harmonic generation imaging.

Semi-automated quantification of filopodial dynamics.

Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells.

Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy.

Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching.

Innate immune-mediated neuronal injury consequent to loss of astrocytes.

A guide to accurate measurement of diffusion using fluorescence correlation techniques with blinking quantum dot nanoparticle labels.

Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP.

Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.

Stoichiometry of molecular complexes at adhesions in living cells.

Beyond photobleaching, laser illumination unbinds fluorescent proteins.

A common mechanism underlies the dark fraction formation and fluorescence blinking of quantum dots.

Probing synaptic signaling with quantum dots.

Second harmonic generation microscopy to investigate collagen configuration: a pericarditis case study.

CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin.

Fluorescence microscopy investigations of ligand propagation and accessibility under adherent cells.

Live-cell visualization of dynamics of HIV budding site interactions with an ESCRT component.

Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis.

Quantification of receptor tyrosine kinase transactivation through direct dimerization and surface density measurements in single cells.

Morphological and functional characterization of cholinergic interneurons in the dorsal horn of the mouse spinal cord.

Enhanced Ca2+ entry due to Orai1 plasma membrane insertion increases IL-8 secretion by cystic fibrosis airways.

Probing the "dark" fraction of core-shell quantum dots by ensemble and single particle pH-dependent spectroscopy.

Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.

Nonlinear laser scanning microscopy of human vocal folds.

Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen.

Determination of membrane protein transporter oligomerization in native tissue using spatial fluorescence intensity fluctuation analysis.

The integrin-ligand interaction regulates adhesion and migration through a molecular clutch.

STICCS reveals matrix-dependent adhesion slipping and gripping in migrating cells.

Quantification of receptor tyrosine kinase activation and transactivation by G-protein-coupled receptors using spatial intensity distribution analysis (SpIDA).

Microstructural characterization of vocal folds toward a strain-energy model of collagen remodeling.

Optimization of malaria detection based on third harmonic generation imaging of hemozoin.

Image correlation spectroscopy for measurements of particle densities and colocalization.

Measuring ligand-receptor binding kinetics and dynamics using k-space image correlation spectroscopy.

Fluctuation imaging spiced up with a piece of PIE.

Spatial intensity distribution analysis (SpIDA): a new tool for receptor tyrosine kinase activation and transactivation quantification.

A nu-space for ICS: characterization and application to measure protein transport in live cells.

Bridging the gap between single molecule and ensemble methods for measuring lateral dynamics in the plasma membrane.

Bone ablation without thermal or acoustic mechanical injury via a novel picosecond infrared laser (PIRL).