Raymond J. Owens

Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis.

Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 A resolution.

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.

Lysine methylation as a routine rescue strategy for protein crystallization.

Analysis of variable N-glycosylation site occupancy in glycoproteins by liquid chromatography electrospray ionization mass spectrometry.

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.

Glycoprotein structural genomics: solving the glycosylation problem.

The structure and transcriptional analysis of a global regulator from Neisseria meningitidis.

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues.

Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 Angstrom resolution.

Semi-automated microseeding of nanolitre crystallization experiments.

Protein production and purification.

Structure of the cold-shock domain protein from Neisseria meningitidis reveals a strand-exchanged dimer.

Expression, purification and crystallization of a lyssavirus matrix (M) protein.

Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P).

The crystal structure of UMP kinase from Bacillus anthracis (BA1797) reveals an allosteric nucleotide-binding site.

A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells.

Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis.

Crystallization and preliminary X-ray analysis of CrgA, a LysR-type transcriptional regulator from pathogenic Neisseria meningitidis MC58.

Generation of baculovirus vectors for the high-throughput production of proteins in insect cells.

The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning.

The production of glycoproteins by transient expression in Mammalian cells.

Rhabdovirus matrix protein structures reveal a novel mode of self-association.

The structure of NMB1585, a MarR-family regulator from Neisseria meningitidis.

The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators.

Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL.

Genetic modification of a baculovirus vector for increased expression in insect cells.

Crystal structure of a novel conformational state of the flavivirus NS3 protein: implications for polyprotein processing and viral replication.

Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling.

Structure and functionality in flavivirus NS-proteins: perspectives for drug design.

Recent advances in the production of proteins in insect and mammalian cells for structural biology.

The crystal structure of NGO0477 from Neisseria gonorrhoeae reveals a novel protein fold incorporating a helix-turn-helix motif.

Improved expression of secreted and membrane-targeted proteins in insect cells.

The structure of a reduced form of OxyR from Neisseria meningitidis.

Structural and functional insights of RANKL-RANK interaction and signaling.

Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains.

Crystal structures of penicillin-binding protein 3 from Pseudomonas aeruginosa: comparison of native and antibiotic-bound forms.

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.

xtalPiMS: a PiMS-based web application for the management and monitoring of crystallization trials.

Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells.

Structure of New Delhi metallo-β-lactamase 1 (NDM-1).

Streamlining homogeneous glycoprotein production for biophysical and structural applications by targeted cell line development.

In situ macromolecular crystallography using microbeams.

Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis.

Outrunning free radicals in room-temperature macromolecular crystallography.

Structural and functional characterization of the kindlin-1 pleckstrin homology domain.

Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118.

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.

S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA.

Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment.

Assay platform for clinically relevant metallo-β-lactamases.

Binding of (5S)-penicilloic acid to penicillin binding protein 3.

A streamlined implementation of the glutamine synthetase-based protein expression system.

Chromophore-linked substrate (CLS405): probing metallo-β-lactamase activity and inhibition.

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications.

Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins.

GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System.