Keiji Itaka

Recent development of nonviral gene delivery systems with virus-like structures and mechanisms.

3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells.

Intratracheal gene transfer of adrenomedullin using polyplex nanomicelles attenuates monocrotaline-induced pulmonary hypertension in rats.

Intranuclear fluorescence resonance energy transfer analysis of plasmid DNA decondensation from nonviral gene carriers.

Polyplex micelles with cyclic RGD peptide ligands and disulfide cross-links directing to the enhanced transfection via controlled intracellular trafficking.

A nanoparticle system specifically designed to deliver short interfering RNA inhibits tumor growth in vivo.

Polyplex nanomicelle promotes hydrodynamic gene introduction to skeletal muscle.

Polyunsaturated fatty acids selectively suppress sterol regulatory element-binding protein-1 through proteolytic processing and autoloop regulatory circuit.

Biodegradable polyamino acid-based polycations as safe and effective gene carrier minimizing cumulative toxicity.

Antiangiogenic gene therapy of solid tumor by systemic injection of polyplex micelles loading plasmid DNA encoding soluble flt-1.

Efficient delivery of bioactive antibodies into the cytoplasm of living cells by charge-conversional polyion complex micelles.

Enhanced transfection with silica-coated polyplexes loading plasmid DNA.

Combination of chondroitin sulfate and polyplex micelles from Poly(ethylene glycol)-poly{N'-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} block copolymer for prolonged in vivo gene transfection with reduced toxicity.

Odd-even effect of repeating aminoethylene units in the side chain of N-substituted polyaspartamides on gene transfection profiles.

Enhanced gene expression promoted by the quantized folding of pDNA within polyplex micelles.

Progress and prospects of polyplex nanomicelles for plasmid DNA delivery.

Enhanced stability and gene silencing ability of siRNA-loaded polyion complexes formulated from polyaspartamide derivatives with a repetitive array of amino groups in the side chain.

PEGylated polyplex with optimized PEG shielding enhances gene introduction in lungs by minimizing inflammatory responses.

Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors.

Effective transgene expression without toxicity by intraperitoneal administration of PEG-detachable polyplex micelles in mice with peritoneal dissemination.

In vivo messenger RNA introduction into the central nervous system using polyplex nanomicelle.

Block/homo polyplex micelle-based GM-CSF gene therapy via intraperitoneal administration elicits antitumor immunity against peritoneal dissemination and exhibits safety potentials in mice and cynomolgus monkeys.

Glycogen shortage during fasting triggers liver-brain-adipose neurocircuitry to facilitate fat utilization.

An injectable spheroid system with genetic modification for cell transplantation therapy.

Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors.

Muscle-targeted hydrodynamic gene introduction of insulin-like growth factor-1 using polyplex nanomicelle to treat peripheral nerve injury.

Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors.

Modulated protonation of side chain aminoethylene repeats in N-substituted polyaspartamides promotes mRNA transfection.

Intrathecal injection of a therapeutic gene-containing polyplex to treat spinal cord injury.

Treatment of neurological disorders by introducing mRNA in vivo using polyplex nanomicelles.

A tadpole-shaped gene carrier with distinct phase segregation in a ternary polymeric micelle.

Gene transfection to spheroid culture system on micropatterned culture plate by polyplex nanomicelle: a novel platform of genetically-modified cell transplantation.

Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity.