Robert Kourist

A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis.

Highly enantioselective kinetic resolution of two tertiary alcohols using mutants of an esterase from Bacillus subtilis.

Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols.

Understanding promiscuous amidase activity of an esterase from Bacillus subtilis.

Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase.

Enzymatic synthesis of optically active tertiary alcohols: expanding the biocatalysis toolbox.

The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering.

Engineering the promiscuous racemase activity of an arylmalonate decarboxylase.

Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases.

Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries.

One-step enzyme extraction and immobilization for biocatalysis applications.

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Biocatalytic synthesis of optically active tertiary alcohols.

Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols.

Creation of a lipase highly selective for trans fatty acids by protein engineering.

The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity.

Photobiocatalytic decarboxylation for olefin synthesis.

A new class of enzymes discovered: a non-heme oxidase produces medium-chain 1-alkenes.

Photosynthetic production of enantioselective biocatalysts.

Draft Genome Sequence of Bordetella bronchiseptica KU1201, the First Isolation Source of Arylmalonate Decarboxylase.

STD-NMR-Based Protein Engineering of the Unique Arylpropionate-Racemase AMDase G74C.

Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance.

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems.

A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts.

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases.

Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water.

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids.