Hidde L. Ploegh

Membrane-specific, host-derived factors are required for US2- and US11-mediated degradation of major histocompatibility complex class I molecules.

The cell biology of antigen presentation.

A closer look at proteolysis and MHC-class-II-restricted antigen presentation.

US2, a human cytomegalovirus-encoded type I membrane protein, contains a non-cleavable amino-terminal signal peptide.

Specific role for cathepsin S in the generation of antigenic peptides in vivo.

Uncoating ATPase Hsc70 is recruited by invariant chain and controls the size of endocytic compartments.

Don Craig Wiley (1944-2001).

Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein.

Human cytomegalovirus gene products US2 and US11 differ in their ability to attack major histocompatibility class I heavy chains in dendritic cells.

Kaposi's sarcoma-associated herpesvirus K3 utilizes the ubiquitin-proteasome system in routing class major histocompatibility complexes to late endocytic compartments.

Neuronal loss and brain atrophy in mice lacking cathepsins B and L.

The human cytomegalovirus US8 glycoprotein binds to major histocompatibility complex class I products.

Proteolysis and antigen presentation by MHC class II molecules.

Analysis of protease activity in live antigen-presenting cells shows regulation of the phagosomal proteolytic contents during dendritic cell activation.

T-cell engagement of dendritic cells rapidly rearranges MHC class II transport.

MHC class I antigen processing regulated by cytosolic proteolysis-short cuts that alter peptide generation.

Lessons from viral manipulation of protein disposal pathways.

Class I negative CD8 T cells reveal the confounding role of peptide-transfer onto CD8 T cells stimulated with soluble H2-Kb molecules.

The human cytomegalovirus US10 gene product delays trafficking of major histocompatibility complex class I molecules.

Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family.

The ubiquitin-proteasome pathway in thymocyte apoptosis: caspase-dependent processing of the deubiquitinating enzyme USP7 (HAUSP).

Invariant chain controls the activity of extracellular cathepsin L.

Mouse MHC class I tetramers that are unable to bind to CD8 reveal the need for CD8 engagement in order to activate naive CD8 T cells.

Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions.

Protein unfolding is not a prerequisite for endoplasmic reticulum-to-cytosol dislocation.

A role for N-glycanase in the cytosolic turnover of glycoproteins.

The caspase-like sites of proteasomes, their substrate specificity, new inhibitors and substrates, and allosteric interactions with the trypsin-like sites.

Ubiquitinylation of the cytosolic domain of a type I membrane protein is not required to initiate its dislocation from the endoplasmic reticulum.

Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy.

Dislocation of a type I membrane protein requires interactions between membrane-spanning segments within the lipid bilayer.

Functional proteomics of the active cysteine protease content in Drosophila S2 cells.

A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L.

Elimination in vivo of developing T cells by natural killer cells.

The ER-lumenal domain of the HHV-7 immunoevasin U21 directs class I MHC molecules to lysosomes.

Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins.

Thymic selection and peripheral activation of CD8 T cells by the same class I MHC/peptide complex.

Yeast N-glycanase distinguishes between native and non-native glycoproteins.

Zinc-finger protein A20, a regulator of inflammation and cell survival, has de-ubiquitinating activity.

A glycosylated type I membrane protein becomes cytosolic when peptide: N-glycanase is compromised.

Dissection of the dislocation pathway for type I membrane proteins with a new small molecule inhibitor, eeyarestatin.

Control of cross-presentation during dendritic cell maturation.

Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells.

Differential dependence of CD4+CD25+ regulatory and natural killer-like T cells on signals leading to NF-kappaB activation.

Chemistry-based functional proteomics: mechanism-based activity-profiling tools for ubiquitin and ubiquitin-like specific proteases.

Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases.

Immunology. Nothing 'gainst time's scythe can make defense...

A membrane protein required for dislocation of misfolded proteins from the ER.

Translating cell biology in vitro to immunity in vivo.

Preferred in vivo ubiquitination sites.

Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment.

Identification of proteins associated with murine cytomegalovirus virions.

Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.

Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover.

Cotranslational endoplasmic reticulum assembly of FcepsilonRI controls the formation of functional IgE-binding receptors.

Small-molecule inhibitors and probes for ubiquitin- and ubiquitin-like-specific proteases.

CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance.

Human cytomegalovirus protein US11 provokes an unfolded protein response that may facilitate the degradation of class I major histocompatibility complex products.

Activity probe for in vivo profiling of the specificity of proteasome inhibitor bortezomib.

Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice.

Cellular internalization of cytolethal distending toxin: a new end to a known pathway.

A molecule's right to choose: how diabetogenic class II MHC products bind peptides.

XBP-1 specifically promotes IgM synthesis and secretion, but is dispensable for degradation of glycoproteins in primary B cells.

A deubiquitinating enzyme encoded by HSV-1 belongs to a family of cysteine proteases that is conserved across the family Herpesviridae.

Effects of PS-341 on the activity and composition of proteasomes in multiple myeloma cells.

Viral modulation of antigen presentation: manipulation of cellular targets in the ER and beyond.

Cathepsin L is essential for onset of autoimmune diabetes in NOD mice.

Ubiquitin--conserved protein or selfish gene?

Multiprotein complexes that link dislocation, ubiquitination, and extraction of misfolded proteins from the endoplasmic reticulum membrane.

Loss of Usp14 results in reduced levels of ubiquitin in ataxia mice.

A deubiquitinating activity is conserved in the large tegument protein of the herpesviridae.

Rapid turnover of unspliced Xbp-1 as a factor that modulates the unfolded protein response.

Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification.

Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: synthesis of active site-directed probes.

Behavioral responses of epidermal Langerhans cells in situ to local pathological stimuli.

Monovalent ligation of the B cell receptor induces receptor activation but fails to promote antigen presentation.

Crystal structure of the boronic acid-based proteasome inhibitor bortezomib in complex with the yeast 20S proteasome.

Regulation of monoubiquitinated PCNA by DUB autocleavage.

A microengraving method for rapid selection of single cells producing antigen-specific antibodies.

High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: mutant viruses altered in its active-site cysteine or histidine are viable.

Signal peptide peptidase is required for dislocation from the endoplasmic reticulum.

Development of intravital intermittent confocal imaging system for studying Langerhans cell turnover.

Chlamydia trachomatis-derived deubiquitinating enzymes in mammalian cells during infection.

Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those of human embryonic stem cell lines.

Murine polyomavirus requires the endoplasmic reticulum protein Derlin-2 to initiate infection.

Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum.

SEL1L, the homologue of yeast Hrd3p, is involved in protein dislocation from the mammalian ER.

Recruitment of CD63 to Cryptococcus neoformans phagosomes requires acidification.

Viral interference with B7-1 costimulation: a new role for murine cytomegalovirus fc receptor-1.

Antigen presentation and the ubiquitin-proteasome system in host-pathogen interactions.

Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells.

AID-/-mus-/- mice are agammaglobulinemic and fail to maintain B220-CD138+ plasma cells.

Chemical probes for the rapid detection of Fatty-acylated proteins in Mammalian cells.

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.

ElaD, a Deubiquitinating protease expressed by E. coli.

The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling.

The mouse polyubiquitin gene UbC is essential for fetal liver development, cell-cycle progression and stress tolerance.

Tubulation of class II MHC compartments is microtubule dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells.

Empty class II major histocompatibility complex created by peptide photolysis establishes the role of DM in peptide association.

TLRs bent into shape.

Sequence and structure evolved separately in a ribosomal ubiquitin variant.

A functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection.

A lipid-based model for the creation of an escape hatch from the endoplasmic reticulum.

Screen for ISG15-crossreactive deubiquitinases.

Differential priming of CD8 and CD4 T-cells in animal models of autoimmune hepatitis and cholangitis.

The peptide cargo of class I molecules: not just passive passengers.

Sortagging: a versatile method for protein labeling.

Mechanisms, biology and inhibitors of deubiquitinating enzymes.

N-linked glycosylation does not impair proteasomal degradation but affects class I major histocompatibility complex presentation.

Bridging B cell and T cell recognition of antigen.

Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers.

Lysosomal cysteine and aspartic proteases are heterogeneously expressed and act redundantly to initiate human invariant chain degradation.

UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes.

The known unknowns of antigen processing and presentation.

Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies.

Live imaging of cysteine-cathepsin activity reveals dynamics of focal inflammation, angiogenesis, and polyp growth.

SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins.

Parasite stage-specific recognition of endogenous Toxoplasma gondii-derived CD8+ T cell epitopes.

The CD8 T-cell response against murine gammaherpesvirus 68 is directed toward a broad repertoire of epitopes from both early and late antigens.

Proteolytic cleavage in an endolysosomal compartment is required for activation of Toll-like receptor 9.

Lipid modification of proteins through sortase-catalyzed transpeptidation.

Profiling antibody responses by multiparametric analysis of primary B cells.

A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway.

Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations.

Ubiquitin C-terminal electrophiles are activity-based probes for identification and mechanistic study of ubiquitin conjugating machinery.

A straight path to circular proteins.

Site-specific protein labeling via sortase-mediated transpeptidation.

XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells.

Ubiquitination, ubiquitin-like modifiers, and deubiquitination in viral infection.

Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells.

Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity.

A gammaherpesvirus ubiquitin-specific protease is involved in the establishment of murine gammaherpesvirus 68 infection.

XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis.

Subtilase cytotoxin cleaves newly synthesized BiP and blocks antibody secretion in B lymphocytes.

The otubain YOD1 is a deubiquitinating enzyme that associates with p97 to facilitate protein dislocation from the ER.

Haploid genetic screens in human cells identify host factors used by pathogens.

A structural element within the HUWE1 HECT domain modulates self-ubiquitination and substrate ubiquitination activities.

Characterization and structural studies of the Plasmodium falciparum ubiquitin and Nedd8 hydrolase UCHL3.

The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating arc.

Differential regulation of effector- and central-memory responses to Toxoplasma gondii Infection by IL-12 revealed by tracking of Tgd057-specific CD8+ T cells.

Transnuclear mice with predefined T cell receptor specificities against Toxoplasma gondii obtained via SCNT.

The transmembrane segment of a tail-anchored protein determines its degradative fate through dislocation from the endoplasmic reticulum.

Quality and quantity control at the endoplasmic reticulum.

The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation.

The tetraspanin CD82 is specifically recruited to fungal and bacterial phagosomes prior to acidification.

Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier.

Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes.

Sortase-catalyzed transformations that improve the properties of cytokines.

Enzymatic blockade of the ubiquitin-proteasome pathway.

Granulin is a soluble cofactor for toll-like receptor 9 signaling.

Sortase A as a tool for high-yield histatin cyclization.

Ubiquitin-dependent control of class II MHC localization is dispensable for antigen presentation and antibody production.

Making and breaking peptide bonds: protein engineering using sortase.

Helicobacter pylori cytotoxin-associated gene A (CagA) subverts the apoptosis-stimulating protein of p53 (ASPP2) tumor suppressor pathway of the host.

Global gene disruption in human cells to assign genes to phenotypes by deep sequencing.

Competition by inhibitory oligonucleotides prevents binding of CpG to C-terminal TLR9.

Fungal recognition is mediated by the association of dectin-1 and galectin-3 in macrophages.

Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control.

CD8α(+) dendritic cells are the critical source of interleukin-12 that controls acute infection by Toxoplasma gondii tachyzoites.

Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it.

Protein quality control in the ER: balancing the ubiquitin checkbook.

Road to ruin: targeting proteins for degradation in the endoplasmic reticulum.

Identification of host cell factors required for intoxication through use of modified cholera toxin.

BAT3 guides misfolded glycoproteins out of the endoplasmic reticulum.

Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes.

Accessory molecules for Toll-like receptors and their function.

Cholera toxin activates nonconventional adjuvant pathways that induce protective CD8 T-cell responses after epicutaneous vaccination.