Keiji Numata

In-situ atomic force microscopy observation of enzymatic degradation in poly(hydroxyalkanoic acid) thin films: normal and constrained conditions.

Enzymatic degradation processes of lamellar crystals in thin films for poly[(R)-3-hydroxybutyric acid] and its copolymers revealed by real-time atomic force microscopy.

Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases.

Enzymatic degradation processes of poly[(R)-3-hydroxybutyric acid] and poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] single crystals revealed by atomic force microscopy: effects of molecular weight and second-monomer composition on erosion rates.

Adsorption of biopolyester depolymerase on silicon wafer and poly[(R)-3-hydroxybutyric acid] single crystal revealed by real-time AFM.

Nonspecific hydrophobic interactions of a repressor protein, PhaR, with poly[(R)-3-hydroxybutyrate] film studied with a quartz crystal microbalance.

Adsorption and hydrolysis reactions of poly(hydroxybutyric acid) depolymerases secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto poly[(R)-3-hydroxybutyric acid].

Branched poly(lactide) synthesized by enzymatic polymerization: effects of molecular branches and stereochemistry on enzymatic degradation and alkaline hydrolysis.

Enzymatic degradation of monolayer for poly(lactide) revealed by real-time atomic force microscopy: effects of stereochemical structure, molecular weight, and molecular branches on hydrolysis rates.

Bioengineered silk protein-based gene delivery systems.

Expression, cross-linking, and characterization of recombinant chitin binding resilin.

Mechanism of enzymatic degradation of beta-sheet crystals.

Reinforcing silk scaffolds with silk particles.

Mechanisms of enzymatic degradation of amyloid Beta microfibrils generating nanofilaments and nanospheres related to cytotoxicity.

Surface-patterned electrode bioreactor for electrical stimulation.

Silk-based delivery systems of bioactive molecules.

Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs.

Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides.

Differences in cytotoxicity of β-sheet peptides originated from silk and amyloid β.

Quantifying osteogenic cell degradation of silk biomaterials.

AFM study of morphology and mechanical properties of a chimeric spider silk and bone sialoprotein protein for bone regeneration.

State of water, molecular structure, and cytotoxicity of silk hydrogels.

Spider silk-based gene carriers for tumor cell-specific delivery.

Silk-based nanocomplexes with tumor-homing peptides for tumor-specific gene delivery.

Biosynthesis of polyhydroxyalkanaotes by a novel facultatively anaerobic Vibrio sp. under marine conditions.

Chemoenzymatic synthesis of poly(L-alanine) in aqueous environment.

Biocompatible and biodegradable dual-drug release system based on silk hydrogel containing silk nanoparticles.

Active intermediates of polyhydroxyalkanoate synthase from Aeromonas caviae in polymerization reaction.

Rapid and efficient gene delivery into plant cells using designed peptide carriers.

Biopolymer-based nanoparticles for drug/gene delivery and tissue engineering.

Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate].

Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specificity.

Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803.

Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil.

Proteinase K-catalyzed synthesis of linear and star oligo(L-phenylalanine) conjugates.

Short one-pot chemo-enzymatic synthesis of L-lysine and L-alanine diblock co-oligopeptides.

Pathway-level acceleration of glycogen catabolism by a response regulator in the cyanobacterium Synechocystis species PCC 6803.

Phasin proteins activate Aeromonas caviae polyhydroxyalkanoate (PHA) synthase but not Ralstonia eutropha PHA synthase.

Silk-pectin hydrogel with superior mechanical properties, biodegradability, and biocompatibility.

Local gene silencing in plants via synthetic dsRNA and carrier peptide.

Synthesis of adhesive peptides similar to those found in blue mussel (Mytilus edulis) using papain and tyrosinase.

Synthesis of homopolypeptides by aminolysis mediated by proteases encapsulated in silica nanospheres.

Recent advances in chemoenzymatic peptide syntheses.

Optimization of poly(N-isopropylacrylamide) as an artificial amidase.

Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers.

Co-expression of two polyhydroxyalkanoate synthase subunits from Synechocystis sp. PCC 6803 by cell-free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-coenzyme A.

Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation as Gene Carriers.

Use of extension-deformation-based crystallisation of silk fibres to differentiate their functions in nature.

Chemoenzymatic Synthesis of Oligo(L-cysteine) for Use as a Thermostable Bio-Based Material.

The Benzyl Ester Group of Amino Acid Monomers Enhances Substrate Affinity and Broadens the Substrate Specificity of the Enzyme Catalyst in Chemoenzymatic Copolymerization.

Influence of Water Content on the β-Sheet Formation, Thermal Stability, Water Removal, and Mechanical Properties of Silk Materials.

Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(Leucine Ethyl Ester) Initiator.

Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli.

Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants.

Relationships between physical properties and sequence in silkworm silks.

Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria.

Enzyme-Mimic Peptide Assembly To Achieve Amidolytic Activity.

Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights.

A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater.

Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film.