subpial-adeno-associated-virus-9-aav9-vector-delivery-in-adult-mice

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

The successful development of a subpial adeno-associated virus 9 (AAV9) vector delivery technique in adult rats and pigs has been reported on previously. ...

University of California, San Diego

Stiff-person syndrome is an uncommon disease characterized by muscular rigidity and painful spasms in the axial and limb muscles. We report a 58-year-old woman with stiff-person syndrome undergoing thymectomy under general anesthesia. Before surgery, her medications were 25 mg of diazepam, 2 mg of clonazepam, and 15 mg of gabapentin per day. After epidural catheterization for the postoperative analgesia, general anesthesia was induced and maintained with continuous remifentanil infusion and propofol with target controlled infusion. With train-of-four ratio (TOFR) monitoring by stimulating the ulnar nerve, her trachea was intubated after 0.6mg x kg(-1) of rocuronium intravenous administration. Since then, additional rocuronium was not given for 4 hours. After surgery, she was fully awake and TOFR recovered to 100%, but tidal volume was too low to remove the tracheal tube, and mechanical ventilation was continued in ICU. On the next day, the tracheal tube was removed, and she was discharged from ICU. Because anesthetics may delay the recovery of respiratory function in a patient with stiff-person syndrome, careful assessment of respiratory function is needed at the emergence from general anesthesia.

[Anesthetic management of a patient with stiff-person syndrome undergoing thymectomy].

Guidewire advancement is interrupted by an internal jugular vein valve with a restricted opening: an ultrasound image.

Ultrasound guidance may be a valuable adjunct for pediatric internal jugular vein catheterization. We previously reported a long-axis in plane technique, called the "three-step method", resulting in high success and a low complication rate by novice operators in adult patients. This is the first report of ultrasound-guided internal jugular vein catheterization (US-IJV) using the three-step method in pediatric patients. Fourteen junior residents underwent simulation training, and then participated in a clinical trial. They performed US-IJV in 14 pediatric patients with congenital heart disease before undergoing cardiac surgery under supervision of an experienced clinician. The overall success rate was 93 %, and all catheterizations were performed within two venipunctures. There were no complications associated with the procedure. The three-step method may facilitate pediatric US-IJV even by a novice operator during their first experience.

The three-step method for ultrasound-guided pediatric internal jugular venous catheterization: a clinical trial.

In this study using 3 different PCA devices (Baxter infuser LVBB +PCM 2 ml: Pump B, Coopdech Balloonjector +PCA 3 ml: Pump C, Rakuraku fuser +PCA 3 ml: Pump S), we investigated how easily PCA devices could be handled.

[A Quantitative Verification for Operability of Three PCA Devices Attached to the Disposable Infusion Pumps].

Dynamic variables based on the heart-lung interaction induced by positive pressure ventilation have not been shown to be useful in assessing cardiac preload in pediatric patients.

Takahiro Tadokoro

Neuroregeneration Laboratory,
Department of Anesthesiology,
Neuroregeneration Laboratory, Department of Anesthesiology

University of California, San Diego

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Takahiro Tadokoro

Neuroregeneration Laboratory,Department of Anesthesiology,Neuroregeneration Laboratory, Department of Anesthesiology

University of California, San Diego

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Neuroregeneration Laboratory,
Department of Anesthesiology,
Neuroregeneration Laboratory, Department of Anesthesiology