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Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Virus-like particles (VLPs) have been used as nanocarriers to display foreign epitopes and/or deliver small molecules in the detection and treatment of ...

University of California Davis

Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β-adrenergic receptor (βAR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length βAR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine.

Mo Baikoghli

Department of Molecular and Cellular Biology

University of California Davis

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Mo Baikoghli

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Molecular and Cellular Biology

University of California Davis

Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods

Department of Molecular and Cellular Biology