Jason Moffat

Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pcl8 and Pcl10.

Mammalian Cdk5 is a functional homologue of the budding yeast Pho85 cyclin-dependent protein kinase.

Transcriptional coregulation by the cell integrity mitogen-activated protein kinase Slt2 and the cell cycle regulator Swi4.

Dissection of a complex phenotype by functional genomics reveals roles for the yeast cyclin-dependent protein kinase Pho85 in stress adaptation and cell integrity.

Ac'septin' a signal: kinase regulation by septins.

Late-G1 cyclin-CDK activity is essential for control of cell morphogenesis in budding yeast.

Exploration of essential gene functions via titratable promoter alleles.

A role for the TGFbeta-Par6 polarity pathway in breast cancer progression.

Dual regulation by pairs of cyclin-dependent protein kinases and histone deacetylases controls G1 transcription in budding yeast.

The ubiquitin-activating enzyme E1 as a therapeutic target for the treatment of leukemia and multiple myeloma.

A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.

Selective targeting of neuroblastoma tumour-initiating cells by compounds identified in stem cell-based small molecule screens.

SATB2 augments ΔNp63α in head and neck squamous cell carcinoma.

A comprehensive platform for highly multiplexed mammalian functional genetic screens.

Pooled lentiviral shRNA screening for functional genomics in mammalian cells.

SnapShot: High-throughput sequencing applications.

CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope.

Inhibition of mitochondrial translation as a therapeutic strategy for human acute myeloid leukemia.

COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines.

Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B.

The mixed lineage leukemia (MLL) fusion-associated gene AF4 promotes CD133 transcription.

Essential gene profiles in breast, pancreatic, and ovarian cancer cells.

A versatile lentiviral expression system to identify mammalian protein-protein interactions.

Developmentally programmed nuclear destruction during yeast gametogenesis.

Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress.

Suppression of cancer progression by MGAT1 shRNA knockdown.

Regulation of CD133 by HDAC6 promotes β-catenin signaling to suppress cancer cell differentiation.

A strategy for modulation of enzymes in the ubiquitin system.

MBNL proteins repress ES-cell-specific alternative splicing and reprogramming.

RNA interference screens to uncover membrane protein biology.

Miniature short hairpin RNA screens to characterize antiproliferative drugs.

Integrated genomic, transcriptomic, and RNA-interference analysis of genes in somatic copy number gains in pancreatic ductal adenocarcinoma.

Targeting HER2+ breast cancer cells: lysosomal accumulation of anti-HER2 antibodies is influenced by antibody binding site and conjugation to polymeric nanoparticles.

A negative genetic interaction map in isogenic cancer cell lines reveals cancer cell vulnerabilities.

shRNA kinome screen identifies TBK1 as a therapeutic target for HER2+ breast cancer.

The RhoGEF GEF-H1 is required for oncogenic RAS signaling via KSR-1.

Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes.

Post-translational regulation of CD133 by ATase1/ATase2-mediated lysine acetylation.

CD133-targeted niche-dependent therapy in cancer: a multipronged approach.

The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.

A genome scale overexpression screen to reveal drug activity in human cells.

Measuring error rates in genomic perturbation screens: gold standards for human functional genomics.

A systematic approach to identify novel cancer drug targets using machine learning, inhibitor design and high-throughput screening.

Differential regulation of FGFR3 by PTPN1 and PTPN2.

Coiled-coil domain containing 68 (CCDC68) demonstrates a tumor-suppressive role in pancreatic ductal adenocarcinoma.

RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR.

Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.

CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.

Inhibition of the Mitochondrial Protease ClpP as a Therapeutic Strategy for Human Acute Myeloid Leukemia.

INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity.

Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes.

Extensive mapping of an innate immune network with CRISPR.

A High Through-put Platform for Recombinant Antibodies to Folded Proteins.

Identification of P450 Oxidoreductase as a Major Determinant of Sensitivity to Hypoxia-Activated Prodrugs.

Selection of recombinant anti-SH3 domain antibodies by high-throughput phage display.

Genome-wide RNAi analysis reveals that simultaneous inhibition of specific mevalonate pathway genes potentiates tumor cell death.

Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint.

High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface.

Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells.

Identification of RNF168 as a PML nuclear body regulator.

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.

Functional Genomic Landscape of Human Breast Cancer Drivers, Vulnerabilities, and Resistance.

Automated inference procedure for the determination of cell growth parameters.

Cytokinetic effects of Wee1 disruption in pancreatic cancer.

Pooled screening for antiproliferative inhibitors of protein-protein interactions.

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.

Inhibition of SCF ubiquitin ligases by engineered ubiquitin variants that target the Cul1 binding site on the Skp1-F-box interface.

BAGEL: a computational framework for identifying essential genes from pooled library screens.

The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination through ATM and DNAPK-Mediated γH2AX Formation.

Efficient generation of patient-matched malignant and normal primary cell cultures from clear cell renal cell carcinoma patients: clinically relevant models for research and personalized medicine.

Kin17 facilitates multiple double-strand break repair pathways that govern B cell class switching.

Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.

Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells.

Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors.

SMYD2 lysine methyltransferase regulates leukemia cell growth and regeneration after genotoxic stress.

Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10.

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens.

RNAi screen identifies essential regulators of human brain metastasis-initiating cells.

Emerging and evolving concepts in gene essentiality.

Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies.

The Inhibitory NKR-P1B:Clr-b Recognition Axis Facilitates Detection of Oncogenic Transformation and Cancer Immunosurveillance.

Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48.

Cotargeting Ephrin Receptor Tyrosine Kinases A2 and A3 in Cancer Stem Cells Reduces Growth of Recurrent Glioblastoma.

CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.

The shieldin complex mediates 53BP1-dependent DNA repair.

A synthetic anti-Frizzled antibody engineered for broadened specificity exhibits enhanced anti-tumor properties.

EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.

Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.

Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons.

Protocadherin-1 is essential for cell entry by New World hantaviruses.

Functional genomic characterization of a synthetic anti-HER3 antibody reveals a role for ubiquitination by RNF41 in the anti-proliferative response.

A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations.

Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15.

Yeast Two-Hybrid Analysis for Ubiquitin Variant Inhibitors of Human Deubiquitinases.

Structure-guided design fine-tunes pharmacokinetics, tolerability, and antitumor profile of multispecific frizzled antibodies.

Global Genetic Networks and the Genotype-to-Phenotype Relationship.

Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.

Genetic interaction networks in cancer cells.

Bmi1 regulates human glioblastoma stem cells through activation of differential gene networks in CD133+ brain tumor initiating cells.

Identifying chemogenetic interactions from CRISPR screens with drugZ.

Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and mice.

CRISPR screens are feasible in TP53 wild-type cells.