Photini Sinnis
Johns Hopkins Malaria Research Institute,
W. Harry Feinstone Department of Molecular Microbiology and Immunology,
Bloomberg School of Public Health,
Johns Hopkins Malaria Research Institute, W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health
Johns Hopkins University
The Sinnis Laboratory is part of the Johns Hopkins Malaria Research Institute, committed to the pursuit of basic science research that translates into solutions targeting one of the most important infectious diseases in the world. Malaria, caused by protozoan parasites of the genus Plasmodium, is transmitted to humans by infected Anopheles mosquitoes. The evolution of drug resistance by the parasite, and insecticide resistance by the mosquito, have created an urgent need for new strategies to control and ultimately eradicate this scourge. Our research focuses on the infective stage of the malaria parasite, sporozoites, which are inoculated into the skin by infected mosquitoes. Sporozoites make an impressive journey from the midgut wall of the mosquito where they emerge from oocysts, to their final destination, the mammalian liver. Using biochemical, cell biological, and genetic approaches as well as intravital imaging and proteomics, we aim to understand the molecular interactions between sporozoites and their mosquito and mammalian hosts that enable the parasite to initiate infection. Recently we have started investigation the quantitative dynamics of transmission from mosquito to mammalian host, an understudied yet important area of inquiry if we are to improve our epidemiologic models and define the minimum efficacy required of transmission blocking interventions.
Evidence that mutant PfCRT facilitates the transmission to mosquitoes of chloroquine-treated Plasmodium gametocytes.
The Journal of infectious diseases Jan, 2011 | Pubmed ID: 21288823
Dendritic cells and hepatocytes use distinct pathways to process protective antigen from plasmodium in vivo.
PLoS pathogens Mar, 2011 | Pubmed ID: 21445239
Total and putative surface proteomics of malaria parasite salivary gland sporozoites.
Molecular & cellular proteomics : MCP May, 2013 | Pubmed ID: 23325771
A host GPCR signaling network required for the cytolysis of infected cells facilitates release of apicomplexan parasites.
Cell host & microbe Jan, 2013 | Pubmed ID: 23332153
Proteolytic Cleavage of the Plasmodium falciparum Circumsporozoite Protein Is a Target of Protective Antibodies.
The Journal of infectious diseases Oct, 2015 | Pubmed ID: 25762791
Reversible Conformational Change in the Plasmodium falciparum Circumsporozoite Protein Masks Its Adhesion Domains.
Infection and immunity Oct, 2015 | Pubmed ID: 26169272
Inhibition by stabilization: targeting the Plasmodium falciparum aldolase-TRAP complex.
Malaria journal Aug, 2015 | Pubmed ID: 26289816
Palmitoyl transferases have critical roles in the development of mosquito and liver stages of Plasmodium.
Cellular microbiology Nov, 2016 | Pubmed ID: 27084458
Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics.
PLoS pathogens 04, 2016 | Pubmed ID: 27128092
Overexpression of Plasmodium berghei ATG8 by Liver Forms Leads to Cumulative Defects in Organelle Dynamics and to Generation of Noninfectious Merozoites.
mBio 06, 2016 | Pubmed ID: 27353755
Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes.
Genome research 01, 2017 | Pubmed ID: 28003436
A human monoclonal antibody prevents malaria infection by targeting a new site of vulnerability on the parasite.
Nature medicine 05, 2018 | Pubmed ID: 29554083
A mosquito salivary gland protein partially inhibits Plasmodium sporozoite cell traversal and transmission.
Nature communications 07, 2018 | Pubmed ID: 30046053
Antibody-Mediated Protection against Sporozoites Begins at the Dermal Inoculation Site.
mBio 11, 2018 | Pubmed ID: 30459199
Author Correction: A human monoclonal antibody prevents malaria infection by targeting a new site of vulnerability on the parasite.
Nature medicine Jan, 2019 | Pubmed ID: 30552419
Reply to Vanderberg, "Further Mechanisms and Locations in Which Antisporozoite Antibodies Neutralize Malaria Sporozoites".
mBio 09, 2019 | Pubmed ID: 31506316
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