Sophia Hober

Structure, specificity, and mode of interaction for bacterial albumin-binding modules.

Mutational analysis of the interaction between albumin-binding domain from streptococcal protein G and human serum albumin.

Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner.

Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology.

Escherichia coli single-stranded DNA-binding protein, a molecular tool for improved sequence quality in pyrosequencing.

Engineering streptococcal protein G for increased alkaline stability.

Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).

An improved dual-expression concept, generating high-quality antibodies for proteomics research.

Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand.

Improving the tolerance of a protein a analogue to repeated alkaline exposures using a bypass mutagenesis approach.

Toward pyrosequencing on surface-attached genetic material by use of DNA-binding luciferase fusion proteins.

Genome-based proteomics.

Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts.

Effect of substrate feed rate on recombinant protein secretion, degradation and inclusion body formation in Escherichia coli.

Affinity ligands for industrial protein purification.

A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein.

High-throughput solubility assay for purified recombinant protein immunogens.

A human protein atlas for normal and cancer tissues based on antibody proteomics.

Pyrosequencing: history, biochemistry and future.

Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling.

High-throughput protein purification using an automated set-up for high-yield affinity chromatography.

From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.

A human protein atlas based on antibody proteomics.

Microfluidic analysis of antibody specificity in a compact disk format.

Single-step recovery and solid-phase refolding of inclusion body proteins using a polycationic purification tag.

Multiplexed PrEST immunization for high-throughput affinity proteomics.

The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.

Approaches for systematic proteome exploration.

High-throughput protein purification under denaturating conditions by the use of cation exchange chromatography.

Z(basic)--a novel purification tag for efficient protein recovery.

Affibody-mediated transferrin depletion for proteomics applications.

A web-based tool for in silico biomarker discovery based on tissue-specific protein profiles in normal and cancer tissues.

Development of affinity columns for the removal of high-abundance proteins in cerebrospinal fluid.

Generation and validation of affinity reagents on a proteome-wide level.

A genecentric Human Protein Atlas for expression profiles based on antibodies.

Evaluation of monospecific antibodies: a comparison study with commercial analogs using immunohistochemistry on tissue microarrays.

MAP20, a microtubule-associated protein in the secondary cell walls of hybrid aspen, is a target of the cellulose synthesis inhibitor 2,6-dichlorobenzonitrile.

High-throughput protein production--lessons from scaling up from 10 to 288 recombinant proteins per week.

Automated sample preparation method for mass spectrometry analysis on recombinant proteins.

Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.

The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells.

Editorial: Biotech in the post genomic era.

A global view of protein expression in human cells, tissues, and organs.

Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples.

A small bispecific protein selected for orthogonal affinity purification.

Towards a knowledge-based Human Protein Atlas.

Editorial: Global biotech challenges.

Targeted protein pullout from human tissue samples using competitive elution.

Enhancing the protein production levels in Escherichia coli with a strong promoter.

Surrogate antigens as targets for proteome-wide binder selection.

Parallel production and verification of protein products using a novel high-throughput screening method.

Translational database selection and multiplexed sequence capture for up front filtering of reliable breast cancer biomarker candidates.

Editorial: biotech reviews - keeping up with current developments.

High nuclear RBM3 expression is associated with an improved prognosis in colorectal cancer.

Engineering bispecificity into a single albumin-binding domain.

Covalent immunoglobulin labeling through a photoactivable synthetic Z domain.