Department of Chemical Engineering,
Institute of Biochemical Engineering,
Key Laboratory of Industrial Biocatalysis,
Ministry of Education,
Center for Synthetic & Systems Biology,
Department of Chemical Engineering, Institute of Biochemical Engineering,
Key Laboratory of Industrial Biocatalysis, Ministry of Education
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Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production.
ACS synthetic biology 12, 2017 | Pubmed ID: 28841296
Rapid detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery.
FEMS microbiology letters Aug, 2005 | Pubmed ID: 16006057
A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes.
Biotechnology letters Jul, 2005 | Pubmed ID: 16132849
Biological wastewater treatment by a bioreactor with repeated coupling of aerobes and anaerobes aiming at on-site reduction of excess sludge.
Water science and technology : a journal of the International Association on Water Pollution Research , 2006 | Pubmed ID: 16841729
Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis.
FEMS microbiology letters Oct, 2006 | Pubmed ID: 16978347
[Study on the structure and function of a stable methane-oxidizing mixed microbial consortium].
Wei sheng wu xue bao = Acta microbiologica Sinica Feb, 2007 | Pubmed ID: 17436634
Enhanced ozonation of simulated dyestuff wastewater by microbubbles.
Chemosphere Aug, 2007 | Pubmed ID: 17467036
Enhanced sludge solubilization by microbubble ozonation.
Chemosphere May, 2008 | Pubmed ID: 18328535
Enhanced coagulation of ferric chloride aided by tannic acid for phosphorus removal from wastewater.
Chemosphere May, 2008 | Pubmed ID: 18395769
Analysis of the mechanism of sludge ozonation by a combination of biological and chemical approaches.
Water research Jan, 2009 | Pubmed ID: 18976790
Optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune.
Applied biochemistry and biotechnology Jan, 2010 | Pubmed ID: 19156366
Paraffin oil as a "methane vector" for rapid and high cell density cultivation of Methylosinus trichosporium OB3b.
Applied microbiology and biotechnology Jun, 2009 | Pubmed ID: 19219428
Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process.
Bioresource technology Jun, 2009 | Pubmed ID: 19231169
Application of combined physicochemical and biological processes for enhanced treatment of avermectin fermentation wastewater.
Water science and technology : a journal of the International Association on Water Pollution Research , 2009 | Pubmed ID: 19237772
Progress and perspectives of sludge ozonation as a powerful pretreatment method for minimization of excess sludge production.
Water research Apr, 2009 | Pubmed ID: 19282018
Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase.
Applied microbiology and biotechnology Sep, 2009 | Pubmed ID: 19390851
Correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures.
Analytical biochemistry Jul, 2009 | Pubmed ID: 19394302
Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine.
FEMS microbiology ecology Nov, 2009 | Pubmed ID: 19515201
Systematic analysis of biochemical performance and the microbial community of an activated sludge process using ozone-treated sludge for sludge reduction.
Bioresource technology Nov, 2009 | Pubmed ID: 19515555
Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production.
Biotechnology letters Oct, 2009 | Pubmed ID: 19533026
Changes in biomass activity and characteristics of activated sludge exposed to low ozone dose.
Chemosphere Sep, 2009 | Pubmed ID: 19698970
Alteration of hydrogen metabolism of ldh-deleted Enterobacter aerogenes by overexpression of NAD+-dependent formate dehydrogenase.
Applied microbiology and biotechnology Mar, 2010 | Pubmed ID: 19830418
Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts.
Applied microbiology and biotechnology Apr, 2010 | Pubmed ID: 20012278
Biochemical analysis and kinetic modeling of the thermal inactivation of MBP-fused heparinase I: implications for a comprehensive thermostabilization strategy.
Biotechnology and bioengineering Aug, 2011 | Pubmed ID: 21445884
Bioengineering of the Enterobacter aerogenes strain for biohydrogen production.
Bioresource technology Sep, 2011 | Pubmed ID: 21764301
Luciferase and fluorescent protein as dual reporters analyzing the effect of n-dodecyltrimethylammonium bromide on the physiology of Pseudomonas putida.
Applied microbiology and biotechnology Jan, 2012 | Pubmed ID: 22075632
Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase.
Enzyme and microbial technology Jan, 2011 | Pubmed ID: 22112777
Improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in Enterobacter aerogenes.
Enzyme and microbial technology Feb, 2011 | Pubmed ID: 22112830
Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli.
Applied microbiology and biotechnology May, 2012 | Pubmed ID: 22588503
Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum.
Applied biochemistry and biotechnology Jun, 2012 | Pubmed ID: 22592776
Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection.
Journal of biotechnology Jan, 2013 | Pubmed ID: 23073152
Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols.
Applied and environmental microbiology Apr, 2013 | Pubmed ID: 23354700
Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility.
Microbial cell factories Mar, 2013 | Pubmed ID: 23497261
Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota.
Microbes and environments , 2013 | Pubmed ID: 24240187
Controllable production of low molecular weight heparins by combinations of heparinase I/II/III.
Carbohydrate polymers Jan, 2014 | Pubmed ID: 24299802
Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool.
Applied microbiology and biotechnology Jun, 2014 | Pubmed ID: 24769904
Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community.
Bioresource technology Jan, 2015 | Pubmed ID: 25459816
High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway.
Microbial cell factories Jan, 2015 | Pubmed ID: 25592762
Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
Bioresource technology Jun, 2015 | Pubmed ID: 25780905
Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production.
Journal of bioscience and bioengineering Oct, 2015 | Pubmed ID: 25795571
DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe.
BMC bioinformatics Mar, 2015 | Pubmed ID: 25888481
Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis.
Applied microbiology and biotechnology Jul, 2015 | Pubmed ID: 26025015
Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes.
Bioresource technology Oct, 2015 | Pubmed ID: 26188552
Construction of a linker library with widely controllable flexibility for fusion protein design.
Applied microbiology and biotechnology Jan, 2016 | Pubmed ID: 26394862
Insights into the global regulation of anaerobic metabolism for improved biohydrogen production.
Bioresource technology Jan, 2016 | Pubmed ID: 26476162
Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli.
Metabolic engineering Jan, 2016 | Pubmed ID: 26506462
Bioconversion of methanol to value-added mevalonate by engineered Methylobacterium extorquens AM1 containing an optimized mevalonate pathway.
Applied microbiology and biotechnology Mar, 2016 | Pubmed ID: 26521242
Characterization of a novel Acinetobacter baumannii xanthine dehydrogenase expressed in Escherichia coli.
Biotechnology letters Feb, 2016 | Pubmed ID: 26543035
A study on the effects of linker flexibility on acid phosphatase PhoC-GFP fusion protein using a novel linker library.
Enzyme and microbial technology Feb, 2016 | Pubmed ID: 26777244
Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin.
Carbohydrate polymers Jun, 2016 | Pubmed ID: 27083825
Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria.
Journal of bioscience and bioengineering Oct, 2016 | Pubmed ID: 27150511
Enrichment and characteristics of mixed methane-oxidizing bacteria from a Chinese coal mine.
Applied microbiology and biotechnology Dec, 2016 | Pubmed ID: 27474022
Xanthine dehydrogenase: An old enzyme with new knowledge and prospects.
Bioengineered Nov, 2016 | Pubmed ID: 27537049
Improved production of trans-4-hydroxy-l-proline by chromosomal integration of the Vitreoscilla hemoglobin gene into recombinant Escherichia coli with expression of proline-4-hydroxylase.
Journal of bioscience and bioengineering Jan, 2017 | Pubmed ID: 27567047
Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.
Biotechnology for biofuels , 2016 | Pubmed ID: 27895708
Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply.
Metabolic engineering 01, 2017 | Pubmed ID: 27919791
Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders: A review.
Carbohydrate polymers Mar, 2017 | Pubmed ID: 28115102
Erratum to: Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors.
Biotechnology for biofuels , 2017 | Pubmed ID: 28174602
Metabolic engineering of Escherichia coli cell factory for highly active xanthine dehydrogenase production.
Bioresource technology Dec, 2017 | Pubmed ID: 28610971
Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice.
Carbohydrate polymers Oct, 2017 | Pubmed ID: 28821136
Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl-Induced Liver Fibrosis.
Frontiers in pharmacology , 2017 | Pubmed ID: 28871223
Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up.
Current opinion in biotechnology 04, 2018 | Pubmed ID: 28892667
A rapid and specific colorimetric method for free tryptophan quantification.
Talanta Jan, 2018 | Pubmed ID: 28917797
Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.
Synthetic and systems biotechnology Jun, 2017 | Pubmed ID: 29062964
Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium extorquens AM1.
Applied biochemistry and biotechnology Jul, 2018 | Pubmed ID: 29243041
Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia coli.
Biotechnology journal Jun, 2018 | Pubmed ID: 29500882
MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae.
Metabolic engineering 05, 2018 | Pubmed ID: 29627507
Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution.
Biotechnology journal Jun, 2018 | Pubmed ID: 29729127
Enhanced Production of Crude Violacein from Glucose in Escherichia coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis.
Applied biochemistry and biotechnology Dec, 2018 | Pubmed ID: 29797295
Massilia violaceinigra sp. nov., a novel purple-pigmented bacterium isolated from glacier permafrost.
International journal of systematic and evolutionary microbiology Jul, 2018 | Pubmed ID: 29851375
Structural characterization and in vitro antioxidant activities of chondroitin sulfate purified from Andrias davidianus cartilage.
Carbohydrate polymers Sep, 2018 | Pubmed ID: 29891311
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance.
Nature communications 06, 2018 | Pubmed ID: 29946130
Improved sgRNA design in bacteria via genome-wide activity profiling.
Nucleic acids research 08, 2018 | Pubmed ID: 29982721
Genome-wide screening identifies promiscuous phosphatases impairing terpenoid biosynthesis in Escherichia coli.
Applied microbiology and biotechnology Nov, 2018 | Pubmed ID: 30229323
Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool.
BMC genomics Apr, 2019 | Pubmed ID: 31035925
Dynamics of transcription-translation coordination tune bacterial indole signaling.
Nature chemical biology 04, 2020 | Pubmed ID: 31873224
Prokaryotic communities in multidimensional bottom-pit-mud from old and young pits used for the production of Chinese Strong-Flavor Baijiu.
Food chemistry May, 2020 | Pubmed ID: 31901820
Highly Efficient Capture of Marine Microbial Strains in Seawater Using Bare FeO Magnetic Beads.
Current microbiology Jul, 2020 | Pubmed ID: 32103330
Microbial microdroplet culture system (MMC): An integrated platform for automated, high-throughput microbial cultivation and adaptive evolution.
Biotechnology and bioengineering 06, 2020 | Pubmed ID: 32159223
Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis.
Applied microbiology and biotechnology May, 2020 | Pubmed ID: 32215707
Hybrid nanoparticles with CO-responsive shells and fluorescence-labelled magnetic cores.
Journal of materials chemistry. B Jan, 2014 | Pubmed ID: 32261388
Electrochemically-responsive magnetic nanoparticles for reversible protein adsorption.
Journal of materials chemistry. B Jun, 2016 | Pubmed ID: 32263099
Increased stability and intracellular antioxidant activity of chlorogenic acid depend on its molecular interaction with wheat gluten hydrolysate.
Food chemistry Apr, 2020 | Pubmed ID: 32387948
Empowering a Methanol-Dependent via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System.
Frontiers in bioengineering and biotechnology , 2020 | Pubmed ID: 32733857
Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides.
Scientific reports 08, 2020 | Pubmed ID: 32868795
A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions.
Carbohydrate polymers Dec, 2020 | Pubmed ID: 32933671
Design and construction of chimeric linker library with controllable flexibilities for precision protein engineering.
Methods in enzymology , 2021 | Pubmed ID: 33482990
Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens.
Metabolic engineering 03, 2021 | Pubmed ID: 33493644
Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells.
Nucleic acids research 02, 2021 | Pubmed ID: 33503261
Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1.
Biotechnology journal Jun, 2021 | Pubmed ID: 33595188
Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review.
Food chemistry Aug, 2021 | Pubmed ID: 33743430
[Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Mar, 2021 | Pubmed ID: 33783163
Genome-wide genotype-phenotype associations in microbes.
Journal of bioscience and bioengineering Jul, 2021 | Pubmed ID: 33895083
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution.
Trends in biotechnology Jan, 2022 | Pubmed ID: 33958227
[Functional discovery and production technology for natural bioactive peptides].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology Jun, 2021 | Pubmed ID: 34227301
Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration.
Biotechnology and applied biochemistry Jul, 2021 | Pubmed ID: 34269481
Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure.
Glycoconjugate journal 10, 2021 | Pubmed ID: 34515908
New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen.
Methods in molecular biology (Clifton, N.J.) , 2022 | Pubmed ID: 34709614
A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris.
Applied microbiology and biotechnology Dec, 2021 | Pubmed ID: 34773154
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast.
ACS synthetic biology Jan, 2022 | Pubmed ID: 35089702
Xingjin Jian*,1,2,
Xiaojie Guo*,1,2,3,
Jia Wang4,
Zheng Lin Tan1,2,6,
Xin-hui Xing1,2,5,
Liyan Wang3,
Chong Zhang1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University,
2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University,
3, Luoyang TMAXTREE Biotechnology Co., Ltd.,
4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University,
5Center for Synthetic & Systems Biology, Tsinghua University,
6School of Life Science and Technology, Tokyo Institute of Technology
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