co-culture-transduction-murine-thymocytes-on-delta-like-4-expressing

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Transduced mouse immature thymocytes can be differentiated into T cells&#160;in vitro&#160;using the delta-like 4-expressing bone marrow stromal cell line ...

National Institutes of Health

Michigan State University

Mammary gland neoplasms in macropods are uncommonly reported, and the morphological and immunohistochemical characteristics are incompletely described. The goal of this study was to describe the morphologic features of macropod mammary neoplasms and to determine the molecular subtypes of mammary carcinomas using a panel of antibodies against estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2), p63, smooth muscle actin (SMA), and epidermal growth factor receptor (EGFR). Biopsy and necropsy specimens were examined from 21 macropods with mammary tumors submitted to Northwest ZooPath from 1996 to 2019. In accordance with the histologic classification of canine mammary tumors proposed by Goldschmidt and colleagues, tubulopapillary (2), tubular (10), and comedo-carcinomas (2), adenoma (1), lobular hyperplasia (3), fibroadenomatous hyperplasia (1), and mastitis (2) were diagnosed. Red kangaroos () were most commonly diagnosed with mammary carcinomas (79% of all carcinomas). Seven carcinomas had lymphovascular invasion and 2 also had pulmonary metastases. Six of these 7 carcinomas were classified as grade 3. Immunohistochemistry (IHC) for all antibodies was performed on 9/14 carcinomas, and partial IHC was performed for 3 cases. All 12 carcinomas were immunoreactive for PR, 5 for ER, 9 for EGFR, and none for Her-2. Five of the 9 mammary carcinomas with complete IHC data were classified as luminal A subtype, and 4 were normal-like subtype. Accurate classification of mammary tumors in macropods based on morphology, immunohistological characteristics, and molecular subtype may be helpful in guiding clinical management, prognosis, and potential therapeutic targets.

Morphological and immunohistochemical characterization and molecular classification of spontaneous mammary gland tumors in macropods.

Soft tissue sarcomas (STSs) are common well-described cutaneous neoplasms in many domestic species. Few cutaneous neoplasms have been reported in cervids, other than papilloma virus-induced benign cutaneous fibromas (BCFs). Two wild-caught Alaskan moose (), housed at different North American zoos, were both presented with lameness and a mass. The gross appearance of each mass varied greatly; case 1 had a distinct, exophytic, ulcerated mass near the right carpal joint similar in appearance to a BCF, and case 2 had a pronounced swelling over the left hip. Based on histologic evaluation, both masses were diagnosed as STS. The clinical presentation of these masses included rapid growth and associated lameness; however, gross appearance was markedly different. Histopathology revealed characteristics typical of STS in other species, including spindle-shaped cells arranged in streaming sheets with ovoid nuclei. STS should be included as a differential diagnosis for moose with a cutaneous or subcutaneous soft tissue mass or swelling.

Soft tissue sarcomas in 2 zoo-housed Alaskan moose.

IL-7 is a member of the family of cytokines with four anti-parallel α helixes that bind Type I cytokine receptors. It is produced by stromal cells and is required for development and homeostatic survival of lymphoid cells.

IL-7: Comprehensive review.

Four Ras guanine nucleotide-releasing proteins (RasGRP1 through 4) belong to the family of guanine nucleotide exchange factors (GEFs). RasGRPs catalyze the release of GDP from small GTPases Ras and Rap and facilitate their transition from an inactive GDP-bound to an active GTP-bound state. Thus, they regulate critical cellular responses via many downstream GTPase effectors. Similar to other RasGRPs, the catalytic module of RasGRP1 is composed of the Ras exchange motif (REM) and Cdc25 domain, and the EF hands and C1 domain contribute to its cellular localization and regulation. RasGRP1 can be activated by a diacylglycerol (DAG)-mediated membrane recruitment and protein kinase C (PKC)-mediated phosphorylation. RasGRP1 acts downstream of the T cell receptor (TCR), B cell receptors (BCR), and pre-TCR, and plays an important role in the thymocyte maturation and function of peripheral T cells, B cells, NK cells, mast cells, and neutrophils. The dysregulation of RasGRP1 is known to contribute to numerous disorders that range from autoimmune and inflammatory diseases and schizophrenia to neoplasia. Given its position at the crossroad of cell development, inflammation, and cancer, RASGRP1 has garnered interest from numerous disciplines. In this review, we outline the structure, function, and regulation of RasGRP1 and focus on the existing knowledge of the role of RasGRP1 in leukemia and other cancers.

Tu Chun Hsu

Cytokines and Immunity Section,
Cancer Innovation Laboratory,
National Cancer Institute,
Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute

National Institutes of Health

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Tu Chun Hsu

Cytokines and Immunity Section,Cancer Innovation Laboratory,National Cancer Institute,Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute

National Institutes of Health

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Cytokines and Immunity Section,
Cancer Innovation Laboratory,
National Cancer Institute,
Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute