qualitative-quantitative-analysis-siderophore-production-from

Qualitative and Quantitative Analysis of Siderophore Production from <em>Pseudomonas aeruginosa</em>

Pseudomonas aeruginosa (P. aeruginosa) is known for its production of a diverse range of virulence factors to establish infections in the host. One such ...

The Maharaja Sayajirao University of Baroda

In the present study, biofilm formation was quantified in UTI isolates of  ( = 22) using the crystal violet assay and was categorized into; strong ( = 16), weak ( = 4), and moderate ( = 2) biofilm producers. Further experiments were done using strong ( = 4) and weak ( = 4) biofilm producers. Biofilm formation was greater in Luria broth followed by natural urine and artificial urine on silicone and silicone-coated latex. Cell adhesion and twitching motility were greater in strong biofilm producers. The presence of thick biofilm with an increased number of dead and total number of cells of strong biofilm producers was observed using CLSM. The concentrations of exopolymeric substances (eDNA, protein, and pel polysaccharide) were high in strong biofilm producers. FEG-SEM visualization of biofilm produced by strong biofilm producers showed more cells encased in thick biofilm matrix than weak ones. Overall results provide evidence for increased cell adhesion and twitching motility in strong biofilm producers.

Cell adhesion and twitching motility influence strong biofilm formation in .

Persister cell (PC) is dormant, tolerant to antibiotics, and a transient reversible phenotype. These phenotypes are observed in P. aeruginosa and cause bacterial chronic infection as well as recurrence of biofilm-mediated infection. PC formation requires stringent response and toxin-antitoxin (TA) modules. This study shows the P. aeruginosa PC formation in planktonic and biofilm stages on ceftazidime, gentamicin, and ciprofloxacin treatments. The PC formation was studied using persister assay, flow cytometry using Redox Sensor Green, fluorescence as well as Confocal Laser Scanning Microscopy, and gene expression of stringent response and TA genes. In the planktonic stage, ceftazidime showed a high survival fraction, high redox activity, and elongation of cells was observed followed by ciprofloxacin and gentamicin treatment having redox activity and rod-shaped cells. The gene expression of stringent response and TA genes were upregulated on gentamicin followed by ceftazidime treatment and varied among the isolates. In the biofilm stage, gentamicin and ciprofloxacin showed the biphasic killing pattern, redox activity, gene expression level of stringent response and TA varied across the isolates. Ceftazidime treatment showed higher persister cells in planktonic growth while all three antibiotics were able to induce persister cell formation in the biofilm stage.

Hiral Patel

Department of Microbiology and Biotechnology Centre,
Faculty of Science,
Department of Microbiology and Biotechnology Centre, Faculty of Science

The Maharaja Sayajirao University of Baroda

Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa

Hiral Patel

Department of Microbiology and Biotechnology Centre,Faculty of Science,Department of Microbiology and Biotechnology Centre, Faculty of Science

The Maharaja Sayajirao University of Baroda

Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa

Department of Microbiology and Biotechnology Centre,
Faculty of Science,
Department of Microbiology and Biotechnology Centre, Faculty of Science