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Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells

Human adipose-derived mesenchymal stem cells (ADSCs) can promote the regeneration and reconstruction of various tissues and organs. Recent research ...

Chinese PLA general hospital

Nankai University

Chinese PLA General Hospital

Fluzoparib (PARP inhibitor) showed promising antitumor activity for advanced ovarian cancer in a phase I study. This study aimed to assess the efficacy and safety of fluzoparib in patients with germline -mutated recurrent ovarian cancer.

An Open-label, Multicenter, Single-arm, Phase II Study of Fluzoparib in Patients with Germline  Mutation and Platinum-sensitive Recurrent Ovarian Cancer.

Using Freeman-Tukey Double Arcsine Transformation in Meta-analysis of Single Proportions.

Fatty acid metabolism (FAM) affects the immune phenotype in a metabolically dynamic tumor microenvironment (TME), but the use of FAM-related genes (FAMGs) to predict the prognosis and immunotherapy response of cutaneous melanoma (CM) patients has not been investigated. In this study, we aimed to construct FAM molecular subtypes and identify key prognostic biomarkers in CM.

Identification of fatty acid metabolism-related molecular subtype biomarkers and their correlation with immune checkpoints in cutaneous melanoma.

The ability to form predictions based on recent sensory experience is essential for behavioral adaptation to our ever-changing environment. Predictive encoding represented by neuronal activity has been observed in sensory cortex, but how this neuronal activity is transformed into anticipatory motor behavior remains unclear. Fiber photometry to investigate a corticostriatal projection from the auditory cortex to the posterior striatum during an auditory paradigm in mice, and pharmacological experiments in a task that induces a temporal expectation of upcoming sensory stimuli. We find that the auditory corticostriatal projection relays both sound-evoked stimulus information as well as predictive signals in relation to stimulus timing following rhythmic auditory stimulation. Pharmacological experiments suggest that this projection is required for the initiation of both sound-evoked and anticipatory licking behavior in an auditory associative-learning behavioral task, but not for the general recognition of presented auditory stimuli. This auditory corticostriatal projection carries predictive signals, and the posterior striatum is critical to the anticipatory stimulus-driven motor behavior.

A corticostriatal projection for sound-evoked and anticipatory motor behavior following temporal expectation.

We aimed to quantitatively and systematically elucidate the rationality of the examined variables as independent risk factors for sternal wound infection. We searched databases to screen studies, ascertained the variables to be analysed, extracted the data and applied meta-analysis to each qualified variable. Odds ratios and mean differences were considered to be the effect sizes for binary and continuous variables, respectively. A random-effects model was used for these procedures. The source of heterogeneity was evaluated using a meta-regression. Publication bias was tested by funnel plot and Egger's test, the significant results of which were then calculated using trim and fill analysis. We used a sensitivity analysis and bubble chart to describe their robustness. After screening all variables in the eligible literature, we excluded 55 because only one or no research found them significant after multivariate analysis, leaving 33 variables for synthesis. Two binary variables (age over 65 years, NYHA class >2) and a continuous variable (preoperative stay) were not significant after the meta-analysis. The most robust independent risk factors in our study were diabetes mellitus, obesity, use of bilateral internal thoracic arteries, chronic obstructive pulmonary disease, prolonged surgery time, prolonged ventilation and critical preoperative state, followed by congestive heart failure, atrial fibrillation, renal insufficiency, stroke, peripheral vascular disease and use of an intra-aortic balloon pump. Relatively low-risk factors were emergent/urgent surgery, smoking, myocardial infarction, combined surgery and coronary artery bypass grafting. Sternal wound infection after open-heart surgery is a multifactorial disease. The detected risk factors significantly affected the wound healing process, but some were different in strength. Anything that affects wound healing and antibacterial ability, such as lack of oxygen, local haemodynamic disorders, malnutrition condition and compromised immune system will increase the risk, and this reminds us of comprehensive treatment during the perioperative period.

Yuting Wang

Department of Plastic and Reconstructive Surgery,
the First Medical Centre,
Department of Plastic and Reconstructive Surgery, the First Medical Centre

Chinese PLA general hospital

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells

Yuting Wang

Department of Plastic and Reconstructive Surgery,the First Medical Centre,Department of Plastic and Reconstructive Surgery, the First Medical Centre

Chinese PLA general hospital

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells

Department of Plastic and Reconstructive Surgery,
the First Medical Centre,
Department of Plastic and Reconstructive Surgery, the First Medical Centre