Design and Implementation of a Rat Ex Vivo Lung Perfusion ModelWilliam J. Cleveland *1, Josephine E. Hees *1,2, Claudius Balzer 1,2, Hunter F. Douglas 1, Troy Stevens 3,4,5, Matthias L. Riess 1,6,7
1Anesthesiology, Vanderbilt University Medical Center, 2Anesthesiology, University of Greifswald, 3Department of Physiology and Cell Biology, University of South Alabama, 4Department of Medicine, University of South Alabama, 5Center for Lung Biology, University of South Alabama, 6Anesthesiology, Tennessee Valley Healthcare System Veterans Affairs Medical Center, 7Pharmacology, Vanderbilt University
Ex vivo lungs are useful for a variety of experiments to collect physiological data while excluding the confounding variables of in vivo experiments. Commercial setups are often expensive and limited in the types of data they can collect. We describe a method for building a fully modular setup, adaptable for various study designs.
