Using the Chick Embryo Brain as a Model for In Vivo and Ex Vivo Analyses of Human Glioblastoma Cell BehaviorNicole G. Pastorino 1, Saori Tomatsu 1, Amy Lin 1, Jackson Doerr 1, Zachary Waterman 1, Krisztina Sershen 1, Pulak Ray 2, Analiz Rodriguez 3, Deni S. Galileo 1
1Department of Biological Sciences, University of Delaware, 2Helen F. Graham Cancer Center and Research Institute, Christiana Care, 3Department of Neurosurgery, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences
Chick embryos are used for studying human glioblastoma (GBM) brain tumors in ovo and in ex vivo brain slice co-cultures. GBM cell behavior can be recorded by time-lapse microscopy in ex vivo co-cultures, and both preparations can be analyzed at the experimental endpoint by detailed 3D confocal analysis.
