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Tecnológico de Monterrey

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Antisense Oligonucleotides as a Tool for Prolonged Knockdown of Nuclear lncRNAs in Human Cell Lines

Rogelio Montiel-Manriquez¹, Clementina Castro-Hernández¹, Cristian Arriaga-Canon¹, Luis A. Herrera^1,2

¹Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología-Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, ²Escuela de Medicina y Ciencias de la Salud, Tecnológico de Monterrey

To analyze the function of lncRNAs in time-dependent processes such as chromosomal instability, a prolonged knockdown effect must be achieved. To that purpose, presented here is a protocol that uses modified antisense oligonucleotides to achieve effective knockdown in cell lines for 21 days.

Biology

In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye

Francesca Lazzeri-Barcelo¹, Pierpaolo Ciardo¹, Barbara Leibiger¹, Ingo B. Leibiger¹, Per-Olof Berggren^*1,2,3, Noah Moruzzi^*1

¹The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, ²Tecnológico de Monterrey, ³Diabetes Research Institute, Miller School of Medicine, University of Miami

Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.