High-Quality Brain and Bone Marrow Nuclei Preparation for Single Nuclei Multiome AssaysCarolina Moraes Cabé 1, Sophie Novault 1, Ana Jeemin Choi 2, Valerie Seffer 1, Laura Barrio Cano 1, Valentina Libri 1,3, Milena Hasan 1
1Cytometry and Biomarkers UTechS, C2RT, Institut Pasteur, Université Paris Cité, 2Microenvironment and Immunity Unit, Institut Pasteur, Université Paris Cité, INSERM U1224, 3Istituto Superiore di Sanità
The success of single-cell/single-nuclei transcriptomics and multi-omics largely depends on the quality of cells/nuclei. Therefore, isolating cells/nuclei from tissue and their purification must be highly standardized. This protocol describes the preparation of nuclei from the brain and bone marrow for downstream single-nuclei multiome assay.
