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Phytometrics Co., Ltd.

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana

Rikako Hirata^*1, Momoko Takagi^*2, Yosuke Toda^2,3, Akira Mine¹

¹Graduate School of Agriculture, Kyoto University, ²Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, ³Phytometrics Co., Ltd.

Here, we present a simple method for direct observation and automated measurement of stomatal responses to bacterial invasion in Arabidopsis thaliana. This method leverages a portable stomatal imaging device, together with an image analysis pipeline designed for leaf images captured by the device.