Ex Vivo Culture of Circulating Tumor Cells in the Cerebral Spinal Fluid from Melanoma Patients to Study Melanoma-Associated Leptomeningeal DiseaseVincent Law 1,2, Inna Smalley 3, Brittany R. Evernden 2, Margaret Baldwin 4, Keiran S. M. Smalley 1, Peter A. Forsyth 1,2
1Department of Tumor Biology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 2Department of Neuro-Oncology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 3Department of Cancer Physiology, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute, 4Department of Comparative Medicine, University of South Florida, H. Lee Moffitt Cancer Center & Research Institute
This article describes a protocol for the propagation of cerebral spinal fluid-circulating tumor cells (CSF-CTCs) collected from patients with melanoma-associated leptomeningeal disease (M-LMD) to develop preclinical models to study M-LMD.
