Medical Faculty of the University of Heidelberg

2 ARTICLES PUBLISHED IN JoVE

Medicine

Controlled Reversible Visceral Arterial Ischemia, Venous Congestion and Combined Malperfusion via Midline Laparotomy in Rats

Alexander Studier-Fischer^1,2,3,4, Berkin Özdemir^1,3,4, Karl-Friedrich Kowalewski^2,3,4, Caelan Max Haney^2,3,4,5, Luisa Egen^2,3,4, Maurice Stephan Michel^2,3,4, Markus Mieth¹, Zoltan Czigany¹, Gabriel Alexander Salg¹, Henrik Nienhüser¹, Franck Billmann¹

¹Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, ²Department of Urology and Urosurgery, University Medical Center Mannheim, Medical Faculty of the University of Heidelberg, ³Division of Intelligent Systems and Robotics in Urology (ISRU), German Cancer Research Center (DKFZ) Heidelberg, ⁴DKFZ Hector Cancer Institute at the University Medical Center Mannheim, ⁵Department of Urology, University Hospital Leipzig

This article introduces a standardized procedure for controlled, reversible malperfusion of visceral organs in rat models. The aim is to induce these malperfusion states with a high degree of methodological certainty and control while maintaining technical simplicity and error resilience.

Medicine

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats

Alexander Studier-Fischer^1,2,3,4, Berkin Özdemir^1,3,4, Caelan Max Haney^2,3,4,5, Luisa Egen^2,3,4, Maurice Stephan Michel^2,3,4, Niklas Westhoff², Markus Mieth¹, Zoltan Czigany¹, Gabriel Alexander Salg¹, Henrik Nienhüser¹, Franck Billmann¹, Karl-Friedrich Kowalewski^2,3,4

This manuscript introduces a standardized protocol for a 5/6 nephrectomy by sharp bipolectomy via midline laparotomy in a rat model, aiming to induce a state of renal insufficiency via renal parenchyma reduction with a great degree of methodical precision and low risk of technical error.