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Meizhou Clinical Institute of Shantou University Medical College

1 ARTICLES PUBLISHED IN JoVE

Biology

Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells

Man Yang¹, Qinghua Liu², Yong Chen², Jun Li², Wen He^1,3

¹Meizhou Clinical Institute of Shantou University Medical College, ²Department of Pathology, Meizhou People's Hospital, ³Guangdong Engineering Technological Research Center of Clinical Molecular Diagnosis and Antibody Drugs, Meizhou Academy of Medical Sciences, Meizhou People's Hospital

Here, we present a protocol to isolate and culture rat endometrial epithelial stem cells (reESCs), generating rat endometrial organoids. This method facilitates in vitro studies of endometrial diseases, enabling gene editing and other cellular manipulations.