Visualization of Neutrophil Extracellular Traps in Mesenteric Venules After Mesenteric Ischemia-Reperfusion Injury via Intravital MicroscopyZhenling Gao 1, Olga Dremova 1, Cornelia Karwot 1, Christoph Reinhardt *1,2, Klytaimnistra Kiouptsi *1,2
1Center for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Johannes Gutenberg-University Mainz, 2German Center for Cardiovascular Research (DZHK), Partner Site Rhine-Main
This protocol presents a method for inducing mesenteric ischemia-reperfusion injury and visualizing neutrophil extracellular traps (NETs) in mesenteric venules using intravital microscopy. Blood flow in the superior mesenteric artery was restricted for 1 h, followed by reperfusion, allowing direct quantification of leukocyte adhesion and NETosis.
