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Tufts School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Alenka Lovy¹, Anthony J.A. Molina², Fernanda M. Cerqueira³, Kyle Trudeau³, Orian S. Shirihai³

¹Department of Neuroscience, Center for Neuroscience Research, Tufts School of Medicine, ²Department of Internal Medicine, Geriatrics & Gerontology, Wake Forest Baptist Medical Center, ³Department of Medicine, Boston University Medical Center

Mitochondrial fusion was measured by tracking the equilibration of photoconverted matrix-targeted GFP across the mitochondrial network over time. Thus far, only one cell could be subjected to an hour long kinetic analysis at a time. We present a method that simultaneously measures multiple cells, thereby speeding up the data collection process.

Biology

Dissection and Immunostaining of Larval Salivary Glands from Anopheles gambiae Mosquitoes

Michelle Z. Chiu^1,2, Steven Lannon¹, Marisol Luchetti¹, Michael B. Wells³, Deborah J. Andrew¹

¹Department of Cell Biology, The Johns Hopkins University School of Medicine, ²Tufts School of Medicine, ³Department of Biomedical Sciences, Idaho College of Osteopathic Medicine

The adult mosquito salivary gland (SG) is required for the transmission of all mosquito-borne pathogens to their human hosts, including viruses and parasites. This video demonstrates efficient isolation of the SGs from the larval (L4) stage Anopheles gambiae mosquitoes and preparation of the L4 SGs for further analysis.