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university of buffalo

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Wei Han¹, Hui Li¹, Brahm H. Segal^2,3, Timothy S. Blackwell¹

¹Department of Medicine, Vanderbilt University School of Medicine, ²Departments of Medicine and Immunology, Roswell Park Cancer Institute, ³Department of Medicine, University at Buffalo School of Medicine

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

Immunology and Infection

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Melissa J. Grimm¹, Anthony C. D'Auria¹, Brahm H. Segal²

¹Department of Medicine, Roswell Park Cancer Institute, ²Department of Medicine, School of Medicine and Biomedical Sciences, University of Buffalo

A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.