Synthesis and Assay of Vibrio Quorum Sensing InhibitorsLaura C. Brown 1, Jay Chopra 2, Rachel E. Horness 3, Julia C. van Kessel 4
1Department of Chemistry, Indiana University, 2Indiana University School of Medicine, 3Department of Chemistry, Carleton College, 4Department of Biology, Indiana University
Thiophenesulfonamide compounds are potent and specific inhibitors of Vibrio quorum sensing regulators LuxR/HapR that block their activity in vivo, thus preventing transcription of genes for virulence, motility, and biofilms. This protocol details how these compounds are synthesized, modeled in silico, and assayed in vivo for activity against LuxR/HapR.
