i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Gabriel S. Oliveira^1,2, Clara M. Bento^1,3,4, António Pombinho^1,3, Rita Reis^1,3, Kevin Van Calster^5,6, Linda De Vooght⁵, André F. Maia^1,3, Paul Cos⁵, Maria Salomé Gomes^1,2, Tânia Silva^1,2

¹i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, ²ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, ³IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, ⁴Programa Doutoral em Biologia Molecular e Celular (MCBiology), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, ⁵Laboratory for Microbiology, Parasitology and Hygiene (LMPH), ⁶Department of Infectious Diseases in Humans, Immune Response, Sciensano

This study developed an assay for high-throughput screening against Mycobacterium abscessus with a recently created double reporter strain, using fluorescence and luminescence to assess bacterial viability quickly. This protocol will be relevant for researchers aiming to screen new drugs against this drug-resistant bacteria.